核和细胞质特定RNA结合蛋白质组的丰富及其在ferroptosis诱导后的变化
Haofan Sun1, Bin Fu1, Xiaohong Qian1
1State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, 102206, China.
Nature communications
|January 29, 2024
概括
这项研究引入了一种新的方法,用于在特定细胞位置上分析RNA结合蛋白 (RBPs). 该技术确定了更多的核和细胞质RBP,揭示了铁灭过程中的动态RBP-RNA相互作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 基因组学就是基因组学.
背景情况:
- RNA结合蛋白 (RBPs) 对于基因表达调节至关重要.
- 它们的功能在很大程度上受到它们在细胞内的位置 (亚细胞局部) 的影响.
- 了解RBP局部化和相互作用是解读基因调节的关键.
研究的目的:
- 开发和验证一种用于亚细胞特异性RNA标记和RBP丰富的方法.
- 以高效率识别核和细胞质RBP.
- 为了研究铁灭过程中RBP-RNA相互作用的变化.
主要方法:
- 开发用于RNA标记的核/细胞质向丰富探针.
- 探测器的应用,以实现RBP的高效丰富和深度分析.
- 在对ferroptosis诱导的反应中对RBP-RNA相互作用的Omics级分析.
- 使用免疫光成像验证已识别的RBP.
主要成果:
- 成功丰富和识别了1221个核和1333个细胞质RBP,显著增加了之前的数量.
- 在铁灭过程中观察到RBP-RNA相互作用的广泛变化,核和细胞质关联增加和减少.
- 确定了许多候选RBP,这些RBP在铁灭过程中在细胞核和细胞质之间转移.
- 在转位RBP中发现了与三碳酸循环相关的蛋白质的丰富.
结论:
- 开发的方法使得RBPs的高效,特定于位置的分析成为可能.
- 铁亡诱导导致RBP-RNA相互作用的显著,位置依赖的变化.
- 候选转位RBP,特别是那些参与三碳酸循环的RBP,可能在铁灭相关的代谢变化中发挥作用.
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