计算机辅助的阿帕特默选择用于制造电化学传感器,以检测阿弗拉托克辛B1
Misgana Mengistu Asmare1,2, Chandran Krishnaraj1,2, Sivaprakasam Radhakrishnan3
1Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, Deokjin-gu, Jeonju-si, Jeollabuk-do, Republic of Korea.
Journal of biomolecular structure & dynamics
|January 30, 2024
概括
对食品安全至关重要的是检测阿弗拉托克辛B1 (AFB1). 这项研究开发了一种使用计算方法和在黄金表面上的固定来进行敏感AFB1检测的DNA吸管生物传感器.
科学领域:
- 生物化学 生物化学
- 纳米技术纳米技术
- 生物传感器是一种生物传感器.
背景情况:
- 非洲毒素B1 (AFB1) 是由阿斯伯吉勒斯菌种产生的强有力的致癌物,对健康构成重大风险.
- 在农产品中早期检测AFB1对于食品安全和公共卫生至关重要.
- 目前的检测方法可能缺乏灵敏度或需要复杂的程序.
研究的目的:
- 通过计算选和识别具有对阿弗拉托克辛B1的高结合亲和度的DNA吸附体.
- 开发和制造一种基于DNA体生物传感器的新型生物传感器,用于敏感的AFB1检测.
- 为了评估制造的生物传感器的分析性能.
主要方法:
- 用分层对接方法和生物信息管道来选ssDNA吸收体对AFB1.1进行查.
- 对所选的体进行了分子动力学 (MD) 模拟,以进行稳定性分析.
- 通过Au-S化学在金面上固定DNA吸管以进行电化学检测.
主要成果:
- 通过MD模拟识别和验证了一种34-mer DNA 合体,其对接得分为-5.959 kcal/mol.
- 制造的DNA-aptamer电极表现出广泛的线性检测范围 (1.0到1000 ng L-1).
- 生物传感器表现出低检测极限 (1.0 ng L-1),高稳定性和出色的可重复性.
结论:
- 计算选有效地确定了AFB1.1的高亲和度DNA吸收体.
- 开发的DNA体生物传感器为AFB1检测提供了一种敏感,稳定和可重复的方法.
- 这种口味传感器有望在食品安全应用中快速可靠地监测AFB1.
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