河马通路中调节Th17分化的分子揭示了结性脊柱炎的严重程度
Xiaohan Xu1, Wanlin Liu2, Yue Liu1
1Department of Rheumatology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
International journal of rheumatic diseases
|January 30, 2024
概括
河马通路调节结性脊髓炎 (AS) 中的T辅助17细胞分化. 上调TAZ和下调MST1/2和NDR1/2蛋白与AS疾病活性有关,并可能提供治疗点.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 类风湿病学 类风湿病学
背景情况:
- 脊髓炎 (AS) 是一种慢性炎症性自身免疫性疾病,影响轴骨架.
- 过度分化的T辅助17 (Th17) 细胞是AS病变发生的关键特征.
- 河马信号通路在与AS相关的Th17分化中的作用尚不清楚.
研究的目的:
- 研究Hippo信号通路在AS患者的炎症Th17分化中的作用.
- 为了检查Hippo通路分子和AS疾病阶段之间的相关性.
主要方法:
- 分析了来自60名AS患者和30名对照组的血液样本.
- 血清中介质素-17 (IL-17) 水平使用ELISA量化.
- 通过实时PCR和Western blot评估了Hippo途径分子 (例如TAZ,MST1/2,NDR1/2) 和RORγt的mRNA和蛋白质表达.
主要成果:
- 在AS患者中,血清IL-17水平升高.
- 在AS患者中观察到RORγt和TAZ的表达增加.
- 发现MST1/2和NDR1/2的水平下降,与TAZ,RORγt和IL-17.0负相关.
结论:
- 河马通路显著调节AS中的Th17分化和疾病活性.
- TAZ上调和MST1/2/NDR1/2下调可能会推动AS的发病.
- 这些分子显示出作为AS的生物标志物和治疗点的潜力.
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