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CsmiR1579-CsKr-h1模块调解了大米干的发育和繁殖:对转基因抗虫大米的有效目标

Yan Tang¹, Shuang Wu¹, Hualiang He¹

¹Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.

International journal of biological macromolecules

|January 30, 2024

概括

一种新型的微RNA,miR-1579,准米干 (Chilo suppressalis) 中的克鲁佩尔同类1 (Kr-h1). 这一发现为控制这一重要的农业害虫提供了一个新的RNAi战略.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
虫害管理虫害管理虫害管理

背景情况:

干 (Chilo suppressalis) 是一种影响大米生产的主要害虫.
在Chilo suppressalis中,抗农药耐药性需要使用替代的控制策略.
微RNA在昆虫的发育和基因调节中起着至关重要的作用.

研究的目的:

为了研究miR-1579在调节基因表达中的作用,在Chilo suppressalis.
探索针对miR-1579进行害虫控制的潜力.
开发一种转基因大米,增强其对Chilo suppressalis的抵抗力.

主要方法:

在体外和体内实验分析miR-1579和克鲁佩尔同类物1 (Kr-h1) 的相互作用.
在Chilo suppressalis幼虫和幼虫中的基因表达分析.
产生和选表达miR-1579.9的转基因大米线.

主要成果:

miR-1579直接结合并负面调节转录因子Kr-h1.
在幼虫中,miR-1579的过度表达减少了生长和生存,并在幼中引起了卵巢异常.
表达miR-1579的转基因大米显著降低了Chilo suppressalis幼虫的生存和损伤.

结论:

miR-1579是Chilos suppressalis中的Kr-h1的一个关键调节剂.
准miR-1579为开发新型害虫控制策略提供了一个有希望的途径.
使用转基因植物的MiRNA介导RNA干扰为控制Chilo suppressalis提供了一种有效的,特定于物种的方法.

关键词:

奇洛 suppressalis 的使用情况.克鲁佩尔的同类1 害虫管理害虫管理害虫管理植物介导的RNAi.miR-157979 的使用情况.

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