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Updated: Jul 4, 2025

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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通过计算性酶工程改善Streptomyces antibioticus中的乙-CoA碳酸酶 (AccB) 的基质结合

Imtiaz Ali1, Dong-Qing Wei2, Abbas Khan2,3

  • 1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China.

Biotechnology and applied biochemistry
|January 30, 2024
PubMed
概括

酶工程发现了三种关键突变 (S343A,T347W,S350W),这些突变显著增强了乙-甲酸碳酸酶 (ACC) 的结合. 这些突变提高了基质结合亲和力,为更高效的多基基和生物燃料的工业生物合成铺平了道路.

关键词:
模拟MD的模拟方法这种菌种是Streptomyces antibioticus.乙烯基-CoA碳酸盐酶是什么炭基转移酶的使用结合基质的基底

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