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上游方法优于密度梯度离心法,在精子加工过程中保护精子DNA完整性

Kenji Amano¹, Satoko Oigawa¹, Koichiro Ichizawa¹

¹Reproduction Center, Toho University Omori Medical Center Tokyo Japan.

Reproductive medicine and biology

|January 30, 2024

概括

与密度梯度离心法相比,上游方法显示了较低的精子DNA碎片化和更高的运动性. 然而,密度梯度离心产生更好的恢复率的渐进性运动精子.

科学领域:

生殖生物学生殖生物学
安德罗学与人类学
精子加工技术精子加工技术

背景情况:

精子DNA碎片化 (SDF) 影响男性生育能力.
精子处理方法,如上游和密度梯度离心 (DGC),用于选择用于辅助生殖技术 (ART) 的运动精子.
评估这些方法对SDF和精子质量的影响对于优化ART结果至关重要.

研究的目的:

为了比较泳起来方法与密度梯度离心法 (DGC) 在减少精子DNA碎片化 (SDF) 的有效性.
评估这些精子处理技术对精子进步运动性和进步运动性精子恢复率的影响.

主要方法:

分析了19个具有高运动精子数量的常态动物精子样本.
精子DNA碎片化 (SDF),渐进性运动性和渐进性运动性精子数量在加工前后都被测量.
样品采用上游法或密度梯度离心法 (DGC) 进行处理.

主要成果:

起初,精子DNA碎片化 (SDF) 在未经加工,游泳或密度梯度离心 (DGC) 样本中没有显著差异.
然而,与DGC加工样本相比,SDF在游起来处理的样本中明显较低.
与未经处理的样本相比,在游起来和DGC处理的样本中,精子的渐进性运动率明显高于未经处理的样本,而游起来显示出优越的运动率.
与DGC相比,在上游方法中,渐进性运动精子的恢复率明显较低.

结论:

游起来方法在减少精子DNA碎片化 (SDF) 和增强精子渐进性运动性方面优越.
密度梯度离心 (DGC) 提供了一个更好的恢复率的渐进性运动精子.
游泳和DGC之间的选择可能取决于精子处理在生育治疗中的具体目标.

关键词:

密度梯度离心法离心法离心法在现场的结端标签.精子DNA碎片化精子DNA碎片化精子的渐进运动性精子的渐进运动性在游泳上游泳.

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