开发,验证和比较一种分析方法,在尿样中同时检测z-药物,使用QuEChERS方法与液体染色学和气体染色学-并联质谱学两种方法
Varsha Chauhan1, Manu Sharma1, Abhishek Tiwari2
1National Forensic Science University, Delhi Campus, Ministry of Home Affairs, Sec-3, Rohini, Delhi 110085, India.
概括
本研究提出了一种经过验证的分析方法,用于检测尿中的z药物,使用QuEChERS样品制备和LC-MS/MS和GC-MS/MS. 该方法可确保用于临床和法医应用的敏感和可靠的检测.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
背景情况:
- Z-药物,镇静剂-催眠剂被滥用,需要用于临床和法医目的的可靠尿液分析.
- 精确检测尿液中的z药物对于患者护理和法律调查至关重要.
研究的目的:
- 开发,验证和比较用于尿液中同时检测z药物的分析方法.
- 优化QuEChERS提取方法,以提高回收率和最小化矩阵效应.
主要方法:
- 使用QuEChERS (快速,轻松,廉价,坚固,有效) 方法进行样本制备.
- 使用液体染色学-并联质谱学 (LC-MS/MS) 和气体染色学-并联质谱学 (GC-MS/MS) 进行同时的z-药物分析.
- 验证了线性,检测极限和量化极限的方法.
主要成果:
- 达到良好的线性 (1-200 ng/mL) 和临床相关的检测/定量限值.
- 无论是LC-MS/MS还是GC-MS/MS,都表现出可靠和一致的结果,准确度和精度高 (>95%).
- 优化的QuEChERS方法提供了高回收率和最小化的矩阵效应.
结论:
- 与QuEChERS合的LC-MS/MS和GC-MS/MS方法提供了一个可靠的解决方案,用于在尿液中同时检测z-药物.
- 在LC-MS/MS和GC-MS/MS之间的选择取决于特定的z-药物和性能要求.
- 这种经过验证的方法适用于临床毒理学,法医分析和z药物监测.
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