培养DPANN古的细胞表面结构Nanobdella aerobiophila
Shingo Kato1, Yuhei O Tahara2, Yuki Nishimura3
1Japan Collection of Microorganisms (JCM), RIKEN BioResource Research Center, Tsukuba, Ibaraki, Japan.
Journal of bacteriology
|January 30, 2024
概括
分析了DPANN archaeon Nanobdella aerobiophila的细胞表面结构,揭示了独特的S层蛋白质和细胞外酶,可能与其宿主Metallosphaera sedula发生ectosymbiosis. 这项研究提出了它们的共生相互作用和细胞周期的模型.
科学领域:
- 微生物学 微生物学
- 考古物 (Archaea) 是一种古老的物种.
- 这种共生是共生.
背景情况:
- DPANN的古老类包括对微生物生态系统至关重要的外共生物种.
- 了解驱动DPANN古老物质外共生的细胞表面机制是有限的.
- 纳诺贝拉有机生物 (Nanobdella aerobiophila) 作为一个模型生物来研究DPANN古老的生态共生.
研究的目的:
- 为了阐明DPANNarchaeon Nanobdella aerobiophila的细胞表面结构和共生机制.
- 研究S层蛋白和细胞外酶在与Metallosphaera sedula的共生相互作用中的作用.
- 提出一种对N. aerobiophila的共生相互作用和细胞周期的模型.
主要方法:
- 利用先进的电子显微镜技术,包括负染色TEM,快速结深蚀刻TEM和3D电子断层扫描.
- 进行了基因组和转录组分析,以确定参与细胞表面结构和共生的关键基因.
- 在 silico 3D 蛋白质结构预测和活细胞成像中使用,以研究蛋白质功能和细胞行为.
主要成果:
- 在N. aerobiophila MJ1T及其宿主M. sedula MJ1HA.之间观察到明显的S层结构.
- 一个高度表达的N. aerobiophila基因编码了一种具有糖化位点和免疫球蛋白类折叠的新型S层蛋白.
- 确定了假定pectin lyase或lectin类细胞外蛋白的基因,这表明它们在共生相互作用中的作用. 实时成像显示了运动细胞复合体,但不是单个N. aerobiophila细胞.
结论:
- 独特的细胞表面结构和N. aerobiophila的新型蛋白质对其外共生生活方式至关重要.
- 一个拟议的模型整合了关于细胞表面特征,基因表达和运动性的发现,以解释共生相互作用和细胞周期.
- 这项研究增强了对DPANN古生物的生理学和生态意义的理解.
更多相关视频
09:12Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Published on: March 10, 2020
7.0K
11:45Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
Published on: June 20, 2018
9.6K
相关概念视频
Prokaryotic Cells
122.5K
Prokaryotes are small unicellular organisms that include the domains—Archaea and Bacteria. Bacteria include many common organisms, such as Salmonella and E. coli, while the Archaea include extremophiles that live in harsh environments, such as volcanic springs.
Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize proteins....
Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize proteins....
122.5K
Cytoskeletal Proteins in Bacteria
3.4K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.4K
Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Membrane Domains
5.4K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
5.4K
