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Updated: Jul 29, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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鉴定了一种新型的热稳定型转氨酶及其在L-phosphinothricin生物合成中的应用

Han-Lin Liu1,2,3, Pu-Hong Yi1,2,3, Jia-Min Wu1,2,3

  • 1Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

Applied microbiology and biotechnology
|January 30, 2024
PubMed
概括

发现了一种新型的恒温转氨酶,用于生产除草剂L-啡三 (L-PPT). 使用这种转氨酶的酶级联有效合成L-PPT并溶解D,L-PPT混合物.

关键词:
不对称的合成方法脱血化 (Deracemization) 是一种使人脱血的过程.酶级联是一种酶级联.这是一种L-phosphinothricin.热稳定的转氨酶酶.

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Production of Pharmaceuticals01:30

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...