反应性氨基功能化微电极阵列提供短期的好处,但长期的损害在vivo记录性能
Brandon S Sturgill1, Ana G Hernandez-Reynoso1, Lindsey N Druschel2,3
1Department of Bioengineering, The University of Texas at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States.
ACS applied bio materials
|January 30, 2024
概括
用 (3-aminopropyl) triethoxysilane (APTES) 涂覆神经记录设备会增加水友性,但会损害长期的神经记录性能,这表明单独使用APTES是有害的.
科学领域:
- 神经科学是一个神经科学.
- 生物材料工程 生物材料工程
- 可植入的设备可以植入设备.
背景情况:
- 皮层内微电极阵列 (MEAs) 对于神经信号记录至关重要.
- 长期植入MEAs会导致神经炎症,降低设备性能和周围组织.
- 生物活性涂层旨在减少神经炎症,通常使用 (3-aminopropyl) triethoxysilane (APTES) 作为链接剂.
研究的目的:
- 调查APTES涂层对MEA的独立影响.
- 为了比较APTES涂层MEA与未涂层MEA的性能和组织反应.
- 评估APTES在16周内对神经记录质量和设备阻抗的影响.
主要方法:
- 用APTES涂覆基板并测量水接触角度.
- 在老鼠运动皮质 (M1) 中植入MEAs用于慢性研究 (16周).
- 评估电化学阻抗,神经记录性能,组织学和基因表达.
主要成果:
- APTES涂层增加了基质的水友性,并在1kHz下降了电化学阻抗.
- 用APTES涂层的MEAs在16周内表现出神经记录性能的一致下降.
- 基因组学和基因表达分析显示,长期组织效应不确.
结论:
- 单独使用的APTES涂层对慢性神经记录性能有害.
- 与APTES的中间功能化步骤可能会对MEA功能产生负面影响.
- 使用APTES的未来研究需要仔细考虑不完整的功能化效应.
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