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在患有过度缩的带膜患者中探索 lncRNA 表达模式

Junling Chen¹, Guibin Zhong², Manle Qiu²

¹Department of Orthopedics, Baoshan Branch, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

|January 31, 2024

概括

这项研究揭示了在带状腺缩 (LFH) 中明显的长非编码RNA (lncRNA) 表达模式,这是脊柱狭窄的原因. lncRNA PARD3-AS1显著影响LFH细胞纤维化,表明其作为治疗点的潜力.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物化学生物化学

背景情况:

带肌缩 (LFH) 是腰椎脊柱狭窄的主要原因,导致患者严重残疾.
长非编码RNAs (lncRNAs) 在LFH病变发生中的作用尚未完全理解,尽管它们已知参与各种生物过程.

研究的目的:

在人体LFH组织中识别差异表达的lncRNA,与非高性带 (LFN) 组织相比.
研究特定的lncRNAs,特别是PARD3-AS1在体外LFH进展中的功能作用.

主要方法:

在人类LFH和LFN样本上进行了lncRNA测序.
定量实时聚合酶连锁反应 (qRT-PCR) 用于验证.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析进行,以探索基因功能. 在体外实验中评估了PARD3-AS1对LFH细胞纤维化的影响.

主要成果:

在LFH组织中共鉴定了1,091种差异表达的lncRNAs,其中645种是上调调的,446种是下调调的.
GO和KEGG分析将这些lncRNA与代谢过程,信号通路 (例如,河马,NF-kappa B) 和DNA修复联系起来.
证实了lncRNA PARD3-AS1的表达,其抑制抑制了LFH细胞纤维化,而其过度表达在体外促进了细胞纤维化.

结论:

不同的lncRNA表达特征与LFH相关,为其分子机制提供了洞察力.
lncRNA PARD3-AS1通过调节纤维化,在LFH的病理生理学中发挥着关键作用,表明其作为治疗点的潜力.

关键词:

过度缩的带肌.长长的非编码RNAs.腰椎脊柱狭窄症腰椎脊柱狭窄症

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...