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对于五种细胞类型的膜透性参数的温度依赖,使用非理想的热力学假设来数学模型冷保护协议

Faranak Yadegari^1,2, Laura A Gabler Pizarro¹, Leah A Marquez-Curtis^1,2

¹Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada.

The journal of physical chemistry. B

|January 31, 2024

概括

这项研究引入了一种新的方法来确定细胞透参数,用于冷保存建模. 这提高了在冷和解期间细胞体积变化的预测,这对于有效的冷保存协议至关重要.

科学领域:

细胞生物学细胞生物学
生物物理学的生物物理.
低温生物学低温生物学

背景情况:

冷保存需要精确控制冷却/加热和冷保护剂 (CPA) 程序,以尽量减少细胞损伤.
细胞体积变化的数学建模对于优化冷保存协议至关重要.
精确的建模需要了解细胞膜对水和CPA的透性,以及透性不活跃的细胞部分.

研究的目的:

开发和验证一种新的两部分配合方法,用于确定关键细胞透参数.
测量5个细胞类型特定的参数,包括水和CPA透性,透性不活性分数和透性病毒系数.
扩展以前的测量技术,包括在0°C的数据,考虑温度依赖的透参数.

主要方法:

通过实验测量平衡和动态细胞体积数据,开发了一种由两部分组成的装配方法.
对五种不同的细胞类型进行了室温和0°C的测量.
该方法结合了非理想溶液热力学,使用了透性维里尔方程.

主要成果:

成功获得了HUVECs,H9c2细胞,PCECs,Jurkat细胞和hCMECs/D3细胞的五个基本细胞类型特异性参数.
新的装配方法有效地解决了技术挑战,并将以前的技术扩展到0°C.
模拟的HUVEC冷保存协议证明了非理想热力学假设对预测细胞体积变化的重大影响.

结论:

开发的方法准确地确定了关键细胞透参数,这对于精确的冷保存建模至关重要.
结合非理想溶液热力学,可以显著改善冷保存过程中细胞体积动态的预测.
这项工作为优化各种细胞类型的冷保存协议提供了一个强大的框架.

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