希斯乳酸化通过调节亚迪波涅的表达方式,参与了牛皮的进展
Sicheng Zhao1, Tingyan Wu1, Mingjing Fu1
1Department of Dermatology, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou, People's Republic of China.
Clinical, cosmetic and investigational dermatology
|January 31, 2024
概括
在牛皮患者中,阿迪波涅丁 (ADIPOQ) 水平降低. 基斯乳化 (H3K18lac) 也下降,抑制了ADIPOQ的转录,并表明了ADIPOQ的存在.
科学领域:
- 生物化学 生物化学
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
背景情况:
- 牛皮是一种慢性炎症性皮肤疾病.
- 阿迪波涅丁 (ADIPOQ) 是脂肪组织中的一个关键蛋白质激素.
- 在牛皮中,希斯乳酸的作用尚不清楚.
研究的目的:
- 为了研究牛皮患者的ADIPOQ表达.
- 为了探索基因素乳酸化对ADIPOQ的缓和作用.
- 为了评估ADIPOQ对牛皮的诊断潜力.
主要方法:
- 分析了GSE78097数据集,用于在牛皮中差异表达的基因.
- 通过Western blot测量了患者皮肤样本中的ADIPOQ,全球乳酸和H3K18lac水平.
- 使用染色体免疫沉 (CHIP) 试验来检查H3K18lac与ADIPOQ促进体区域的结合.
- 进行ROC分析以评估ADIPOQ的诊断价值.
主要成果:
- 在牛皮皮肤组织中,ADIPOQ水平显著下降.
- 在牛皮患者中,全球乳糖和H3K18lac水平也降低了.
- 在体外,增加乳化增强了ADIPOQ蛋白水平,而LDHA抑制降低了它们.
- CHIP测定证实了H3K18lac与ADIPOQ促进器区域的结合.
- ROC分析表明ADIPOQ对牛皮具有诊断价值.
结论:
- 在牛皮中,ADIPOQ的下调,具有诊断潜力.
- 降低H3K18lac水平抑制ADIPOQ转录,有助于降低牛皮中的ADIPOQ.
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