相关实验视频

Updated: Jul 4, 2025

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

Published on: December 4, 2015

猪的基因编辑用于控制流感A病毒感染

Taeyong Kwon¹, Bianca L Artiaga¹, Chester D McDowell¹

¹Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USA.

bioRxiv : the preprint server for biology

|January 31, 2024

概括

基因编辑猪去除跨膜血清蛋白酶2 (TMPRSS2) 显著减少了流感A病毒 (IAV) 复制和疾病. 这提供了一种控制猪体内感染病毒的策略,并防止未来的流行病.

科学领域:

病毒学病毒学
遗传学遗传学是一个
动物科学动物科学

背景情况:

宿主蛋白酶对血凝素 (HA) 的蛋白质分解激活对于流感A病毒 (IAV) 传染性至关重要.
跨膜血清蛋白酶2 (TMPRSS2) 激活具有单基 HA 基因的病毒,与诸如 furin. furin. 等无处不在的蛋白酶不同.
猪是IAV的天然宿主,并作为潜在的流行病菌株的"混合容器".

研究的目的:

为了研究TMPRSS2对大流行H1N1和H3N2 IAV在猪中的复制的影响.
评估缺乏TMPRSS2的基因编辑猪对控制IAV的潜力.

主要方法:

使用CRISPR/Cas9基因编辑来创建同卵性TMPRSS2淘汰赛 (KO) 猪.
在KO猪的初级呼吸道细胞中评估IAV复制 in vitro.
在KO猪体内评估了IAV感染,病毒分泌和病理学,与野生型 (WT) 猪相比.

主要成果:

在TMPRSS2 KO猪呼吸细胞中观察到延迟的IAV复制 in vitro.
在体内,TMPRSS2 KO猪在上呼吸道中病毒分泌减少.
与WT相比,在KO猪中发现了较低的病毒标位和较低的下呼吸道病理病变,在KO猪中发现了较低的病毒标位和较低的下呼吸道病变.

结论:

TMPRSS2在猪的IAV复制和病原发生方面发挥着重要作用.
经过基因编辑的TMPRSS2 KO猪对IAV感染表现出耐药性.
这些发现支持使用基因编辑猪来减轻IAV造成的经济损失,并减少流行病风险.

更多相关视频

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

相关概念视频

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.