相关实验视频

Updated: Jun 10, 2026

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Published on: April 23, 2019

索克斯9将胆道成熟与分支形态发生联系起来

Hannah R Hrncir^1,2, Sergei Bombin¹, Brianna Goodloe¹

¹Department of Medicine, Division of Digestive Diseases, Emory University. Atlanta, GA USA.

bioRxiv : the preprint server for biology

|January 31, 2024

概括

Sox9对于肝脏中小胆管的发展至关重要. 它的缺失会损害胆道上皮细胞的成熟,并导致成年肝脏中的管状细胞减少.

科学领域:

发育生物学是发展生物学.
细胞生物学细胞生物学
肝病学肝病学是一种肝病学.

背景情况:

分支形态发生是组织结构和细胞分化的关键.
肝内胆道 (IHBD) 形态发生涉及胆道上皮细胞 (BEC) 规范,并形成复杂的胆道网络.
了解IBHD发育的调节者对于肝脏生物学来说至关重要.

研究的目的:

调查Sox9在小肝内胆道的发育建立中的作用.
阐明Sox9调节IHBD形态发生和BEC成熟的分子机制.

主要方法:

在小鼠中,Sox9基因的发育性淘汰.
对IHBD形态和分支在产后肝脏的分析.
评估BEC成熟标志物和信号通路 (TGF-β,Activin A) 的评估.
使用BEC有机体模型来研究Activin A的影响.

主要成果:

对于小胆管的发育形成,Sox9是必需的.
Sox9的丧失导致出生后分支形态发生障碍,成年肝脏的管状细胞减少.
缺少Sox9会导致不成熟的BECs,TGF-β信号和Activin A.的提升.
发现Activin A诱导了BEC有机体的发育基因表达和形态缺陷,并抑制了导管的形成.

结论:

在肝脏发育过程中,Sox9在建立小胆管的前体方面发挥着关键作用.
BEC成熟和成年IHBD形态是由Sox9-依赖的导管前体形成调节的.
部分通过Sox9调节的Activin A的下调,对于管状细胞的形成和BEC的成熟很重要.

更多相关视频

Determining Bile Duct Density in the Mouse Liver

Determining Bile Duct Density in the Mouse Liver

Published on: April 30, 2019

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

相关概念视频

Pleiotropy

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...

Maturation of Endosomes

Maturation of Endosomes

The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...

Bone Formation by Endochondral Ossification

Bone Formation by Endochondral Ossification

Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...