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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jul 4, 2025

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
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Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

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基于对齐的HLA类型算法对RNA-seq数据进行了严格的基准测试.

Dottie Yu1, Ram Ayyala1, Sarah Hany Sadek2,3

  • 1Department of Quantitative and Computational Biology, Dornsife College of Letters, Arts and Sciences, University of Southern California, 1975 Zonal Ave, Los Angeles, CA 90033, USA.

bioRxiv : the preprint server for biology
|January 31, 2024
PubMed
概括

这项研究对12个基于RNA-seq的人类白细胞抗原 (HLA) 的类型工具进行了基准测试. 结果将为临床和研究应用指导精确和高效的HLA呼叫器的选择.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
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相关实验视频

Last Updated: Jul 4, 2025

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Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

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科学领域:

  • 基因组学就是基因组学.
  • 免疫遗传学 免疫遗传学
  • 生物信息学是一种生物信息学.

背景情况:

  • 准确的人类白细胞抗原 (HLA) 排型对于移植和药物基因组学至关重要.
  • 许多计算工具现在从RNA测序 (RNA-seq) 数据中归因HLA类型.
  • 这些工具缺乏全面的性能比较,阻碍了知情选择.

结论:

  • 基于RNA-seq的HLA调用器显示出高质量的打字的希望.
  • 当前的工具可能无法在所有祖先之间最佳地平衡精度和计算效率.
  • 这项研究将为选择合适的HLA类型化工具提供必要的指导.