HSV-1 ICP0 Dimer域采用了一个新的β-桶折叠
Erick McCloskey1, Maithri Kashipathy2, Anne Cooper3
1Department of Molecular Biosciences, University of Kansas, Lawrence, KS, USA.
bioRxiv : the preprint server for biology
|January 31, 2024
概括
简单疹病毒1感染细胞蛋白0 (ICP0) 双元域的结构使用X射线结晶学确定. 这种新的结构揭示了对病毒基因调节和复制的洞察力.
科学领域:
- 结构生物学是结构生物学.
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 简单疹病毒1 (HSV-1) 直接早期蛋白 感染细胞蛋白0 (ICP0) 调节病毒基因表达和复制.
- ICP0具有E3泛基因酶活性,其C端二元域 (残留物555-767) 对于交易活化和病毒复制至关重要.
- 双元域的删除会损害病毒基因表达,Lytic感染和从潜伏状态的重新激活.
研究的目的:
- 为了确定ICP0 C终端二元域的三维结构.
- 阐明ICP0的交易活动和在HSV-1复制中的作用的结构基础.
主要方法:
- 从细菌中净化ICP0的C端.
- 进行X射线晶体学以解决蛋白质结构.
- 计算分析以评估蛋白质相互作用.
主要成果:
- ICP0二元域表现出一种新的折叠,以前在结构数据库中没有观察到.
- 每个单体由9个β链和2个α螺旋组成.
- 模聚体的形成涉及β-链的互,形成β-桶结构,这些结构通过堆叠的β-桶进一步组装成四聚体.
- 广泛的键稳定了二元体.
- ICP0可以形成二元体或结合SUMO1,但不是同时.
结论:
- ICP0二元域的确定结构是新的,并为其功能提供了结构基础.
- 了解这种结构可以为ICP0的监管活动提供关键的见解.
- 这些知识将有助于我们更好地了解HSV-1生命周期和潜在的治疗点.
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