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Updated: Jul 4, 2025

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

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在不同蛋白质组中对调节蛋白的高通量识别

Timothy M Locke¹, Rose Fields², Hayden Gizinski¹

¹Department of Pharmacology, University of Washington, Seattle, Washington 98195, United States.

bioRxiv : the preprint server for biology

|January 31, 2024

概括

研究人员使用一种新的热稳定性测定方法在各物种中确定了2884种调节蛋白. 这为了解提供了一个关键资源.

科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
细胞生物学细胞生物学

背景情况:

离子是几乎所有生物过程中的关键调节者.
识别结合的蛋白质 (效应物) 对于理解细胞信号来说至关重要.
目前用于识别结合蛋白的现有方法缺乏高通量,公正和定量能力.

结论:

开发了一种无偏见的,高通量蛋白质学方法,用于识别-蛋白相互作用.
建立了一个全面的资源,用于研究跨物种的信号和调节.
提供了关于结合在各种生物过程中的机械效应的见解.

关键词:

离子的参与是离子的参与.细胞信号传递是细胞的信号传递.多不和脂肪酸的氧化过程.热稳定性蛋白质学热稳定性

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Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...