在Pol II触发环哈普洛类型中的更高阶表观.
Bingbing Duan1, Chenxi Qiu2, Steve W Lockless3
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260.
bioRxiv : the preprint server for biology
|January 31, 2024
概括
RNA聚合酶II的触发循环 (TL) 显示了特定物种的不相容性,揭示了复杂的遗传相互作用. 这些发现揭示了残留物相互作用如何推动这种必不可少的转录机制的演变.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 生物化学 生物化学
背景情况:
- RNA聚合酶II (Pol II) 对于转录至关重要,具有保存的触发环 (TL) 域调节保真性和速度.
- 之前的研究探索了在TL内和周围的双对基因相互作用,以了解残留物功能.
- 在*Saccharomyces cerevisiae* Pol II环境中,观察到来自不同物种的TL之间存在广泛的不兼容性.
研究的目的:
- 调查物种特异性TL序列与*S. cerevisiae* Pol II不相容的原因.
- 在多次替代的TLs中剖析更高阶的遗传相互作用.
- 了解在TL域的演变中表观论的作用.
主要方法:
- 分析TL残留物与周围区域之间的双对基因相互作用.
- 在 *S. cerevisiae* Pol II 系统中构建和分析多重置换的TLs.
- 鉴定和分类由复杂的残留物相互作用引起的表观性模式.
主要成果:
- 鉴定了异种TLs和*S. cerevisiae* Pol II之间的广泛不兼容性,表明了特定物种的表现.
- 揭示了TL单元类型内的积极和消极的上级残留物相互作用.
- 证明复杂的表观症有时仅从中间基因型中显现出来.
结论:
- 复杂的,涉及TL残留物的更高阶表症在Pol II的功能和演变中起着重要作用.
- 特定的TL残留物表现出明显的表观性模式,可能引导TL域的演变.
- 表观相互作用为Pol II的演变提供了机械的见解.
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