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相关概念视频

Epistasis Analysis01:09

Epistasis Analysis

5.0K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
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Epistasis01:39

Epistasis

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In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
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Multiple Allele Traits01:49

Multiple Allele Traits

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The Concept of Multiple Allelism
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Translesion DNA Polymerases02:10

Translesion DNA Polymerases

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Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
10.0K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.1K
Exon Recombination02:32

Exon Recombination

3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
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相关实验视频

Updated: Jul 4, 2025

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
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An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

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在Pol II触发环哈普洛类型中的更高阶表观.

Bingbing Duan1, Chenxi Qiu2, Steve W Lockless3

  • 1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260.

bioRxiv : the preprint server for biology
|January 31, 2024
PubMed
概括
此摘要是机器生成的。

RNA聚合酶II的触发循环 (TL) 显示了特定物种的不相容性,揭示了复杂的遗传相互作用. 这些发现揭示了残留物相互作用如何推动这种必不可少的转录机制的演变.

关键词:
深度突变扫描 (deep mutational scanning) 是一种对突变进行深度扫描的方法.史诗主义就是一种史诗主义.单质类型的单质类型这是一个触发环.

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CRISPR-Mediated Reorganization of Chromatin Loop Structure
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An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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CRISPR-Mediated Reorganization of Chromatin Loop Structure
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CRISPR-Mediated Reorganization of Chromatin Loop Structure

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物化学 生物化学

背景情况:

  • RNA聚合酶II (Pol II) 对于转录至关重要,具有保存的触发环 (TL) 域调节保真性和速度.
  • 之前的研究探索了在TL内和周围的双对基因相互作用,以了解残留物功能.
  • 在*Saccharomyces cerevisiae* Pol II环境中,观察到来自不同物种的TL之间存在广泛的不兼容性.

研究的目的:

  • 调查物种特异性TL序列与*S. cerevisiae* Pol II不相容的原因.
  • 在多次替代的TLs中剖析更高阶的遗传相互作用.
  • 了解在TL域的演变中表观论的作用.

主要方法:

  • 分析TL残留物与周围区域之间的双对基因相互作用.
  • 在 *S. cerevisiae* Pol II 系统中构建和分析多重置换的TLs.
  • 鉴定和分类由复杂的残留物相互作用引起的表观性模式.

主要成果:

  • 鉴定了异种TLs和*S. cerevisiae* Pol II之间的广泛不兼容性,表明了特定物种的表现.
  • 揭示了TL单元类型内的积极和消极的上级残留物相互作用.
  • 证明复杂的表观症有时仅从中间基因型中显现出来.

结论:

  • 复杂的,涉及TL残留物的更高阶表症在Pol II的功能和演变中起着重要作用.
  • 特定的TL残留物表现出明显的表观性模式,可能引导TL域的演变.
  • 表观相互作用为Pol II的演变提供了机械的见解.