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多重固相RPA合CRISPR基于SARS-CoV-2的视觉检测

Xiaochen Qin1, Ratul Paul2, Yuyuan Zhou1

  • 1Department of Bioengineering, Lehigh University, Bethlehem, PA, 18015, USA.

Biosensors & bioelectronics: X
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一种新的spRPA-CRISPR试验提供了SARS-CoV-2的快速视觉检测. 这种敏感的COVID-19测试可以识别低基因度,帮助控制流行病.

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在 COVID-19 疫情中,基于CRISPR的测定方法这是一个DNA传感器DNA传感器.多重复合的spRPA-CRISPR测定方法关怀点的关怀点视觉检测 视觉检测是一种视觉检测.

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科学领域:

  • 生物技术是生物技术.
  • 分子诊断学 分子诊断
  • 公共卫生 公共卫生

背景情况:

  • 由于COVID-19的流行,需要快速,灵敏和易于使用的诊断工具.
  • 目前的监测方法需要改进,以有效控制SARS-CoV-2.

研究的目的:

  • 开发和演示基于CRISPR (spRPA-CRISPR) 的固相异热复合酶聚合酶放大合试验.
  • 为了实现COVID-19DNA的芯片上,复合,敏感和视觉检测.

主要方法:

  • 使用了针对SARS-CoV-2结构蛋白基因的spRPA-CRISPR试验.
  • 在光信号检测中使用Cas12a-crRNA附带裂纹.
  • 集成放大和检测在一个单一的设备上,用于视觉读取.

主要成果:

  • 该试验证明了两个特定的SARS-CoV-2基因的多重检测,没有交叉相互作用.
  • 实现了分析灵敏度降至每微升20个副本.
  • 使用廉价蓝色LED手电筒启用可见的COVID-19检测.

结论:

  • 开发的spRPA-CRISPR平台为多重基因检测提供了一种敏感,可视和易于阅读的方法.
  • 这种测试适用于快速的COVID-19诊断和监测.
  • 检测系统的成本效益性提高了其在全球公共卫生应用中的潜力.