在COVID-19糖尿病患者中,dysbiotic perio-pathogens和炎症发起者和调解者之间的关联
Endang W Bachtiar1,2, Boy M Bachtiar1,2, Ardiana Kusumaningrum3
1Department of Oral Biology, Faculty of Dentistry Universitas Indonesia, Indonesia.
Heliyon
|January 31, 2024
概括
患有糖尿病的COVID-19患者显示了血管酶转化酶-2 (ACE2) 表达和牙周炎症标志物之间的相关性. 这表明ACE2可能会使COVID-19的糖尿病牙周炎患者的炎症恶化.
科学领域:
- 牙周病学 牙周病学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 慢性牙周炎是一种常见的口腔感染.
- 新出现的证据表明,冠状病毒疾病 (COVID-19) 和牙周炎之间存在联系.
- ангиотензин转化酶-2 (ACE2) 是SARS-CoV-2的关键受体,在炎症中起作用.
研究的目的:
- 研究患有牙周炎和COVID-19的患者牙周组织中ACE2的表达.
- 确定在牙周组织中ACE2表达和炎症标志物之间的相关性.
- 评估ACE2在COVID-19背景下加剧牙周炎症中的作用,特别是在糖尿病患者中.
主要方法:
- 使用定量实时PCR (qPCR) 来测量ACE2,IL-6,IL-8,补充C3和LL-37的mRNA表达.
- 下斑块和牙裂液 (GCF) 样本的分析.
- 使用关键牙周病原体 (Porphyromonas gingivalis,Fusobacterium nucleatum,Veillonella parvula) 的比例来评估细菌失生症.
- 接受器操作特征 (ROC) 和曲线下的面积 (AUC) 分析进行,以评估炎症指标的诊断潜力.
主要成果:
- 在所有患者群体中检测到ACE2.
- 在ACE2表达和炎症标志物之间的显著正相关性仅在COVID-19糖尿病患者 (组1) 中观察到.
- IL-6和C3mRNA水平的组合显示了牙周炎症的预测潜力,与第2组 (0.55) 相比,第1组 (0.78) 的AUC值更高.
结论:
- 在COVID-19的糖尿病患者中,ACE2表达与牙周炎症有关.
- IL-6和补充C3之间的相互作用可以作为这个患者群体中牙周炎症严重程度的预测指标.
- 这些发现突出了一个潜在的机制,将COVID-19,糖尿病和牙周病恶化联系在一起.
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