在患有慢性乙型肝炎的患者中,血清乙型肝炎病毒拼接RNA的比例随着肝病的进展而增加

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Updated: Jul 4, 2025

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Xingyu Lu¹, Wanying Li¹, Rui Deng¹

¹State Key Laboratory of Organ Failure Research; Key Laboratory of Infectious Diseases Research in South China, Ministry of Education; Guangdong Provincial Key Laboratory of Viral Hepatitis Research; Guangdong Provincial Clinical Research Center for Viral Hepatitis; Guangdong Institute of Hepatology; Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Journal of medical virology

|January 31, 2024

概括

血清乙型肝炎病毒拼接RNAs (spRNAs) 表明慢性乙型肝炎患者的肝病进展和肝硬化风险较高. 测量血清spRNA可能有助于监测疾病并指导CHB治疗策略.

科学领域:

肝病学肝病学是一种肝病学.
病毒学病毒学
分子生物学分子生物学

背景情况:

血清乙型肝炎病毒拼接RNAs (spRNAs) 在HBV感染的患者中存在,但其临床相关性尚不清楚.
慢性乙型肝炎 (CHB) 是一个重要的全球健康问题,肝病的进展和肝硬化是主要的并发症.

研究的目的:

研究血清HBVspRNAs与CHB患者肝病进展之间的关联.
通过体外细胞系实验探索spRNAs在肝纤维化中的作用.

主要方法:

在279名先前未接受治疗的HBeAg阳性CHB患者中,量化了血清HBV野生型RNA (wtRNA) 和spRNA水平.
通过下一代测序确定了spRNA比例和描述的spRNA物种.
进行了体外实验,以评估spRNA对肝脏星体细胞的影响.

主要成果:

在HBV基因型C患者中,与基因型B患者相比,spRNA比例显著更高 (1.76%对比0.84%,p < 0.001).
增加的spRNA比例与更高的阿兰氨酸转移酶水平相关,并且在肝硬化患者中显著增加 (1.69%对1.04%,p = 0.001).
多变量分析显示,高spRNA比例 (p = 0.024) 增加了肝硬化风险的2.566倍;体外研究将spRNA与肝星细胞激活联系起来.

结论:

血清HBV spRNA表达量的增加是CHB肝病进展的危险因素.
血清HBVspRNAs的量化可以帮助监测疾病进展.
针对HBV spRNAs进行治疗可能会提供一种新的方法来改善CHB患者的治疗结果.

关键词:

乙型肝炎病毒的感染.肝脏疾病是一种肝脏疾病.量化量化量化量化量化量化量化量化量化量化量化量化量拼接的RNA是指拼接的RNA.野生类型的RNA.

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