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通过代谢工程和细菌-酵母培养,新兴合成二-β-离子

Zhipeng Qi^1,2,3, Xinyi Tong^1,2, Kaixuan Ke^2,4

¹Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, 159 Long Pan Road, Nanjing 210037, China.

Journal of agricultural and food chemistry

|January 31, 2024

概括

使用工程Escherichia coli和Saccharomyces cerevisiae实现了二-β-离子子的微生物生产. 这种可持续的生物工艺通过一种新的共同培养策略将二-β-离子子产量提高到27mg/L.

科学领域:

合成生物学合成生物学
代谢工程是代谢工程.
工业生物技术工业生物技术

背景情况:

二-β-离子子是关键的风味和香味化合物.
微生物生产为天然提取提供了一个可持续的替代方案.
对于二-β-离子子的本源生物合成途径还没有得到很好的定义.

研究的目的:

为了建立一个新的微生物合成途径dihydro-β-ionone.
为了设计大肠杆菌以生产二-β-离子子.
通过共同培养系统提高二-β-离子子产量.

主要方法:

在大肠杆菌中重建二-β-离子子子通路.
代谢工程策略,以优化前体转化.
使用Saccharomyces cerevisiae进行全细胞催化,将β-离子子转化为二-β-离子子.
开发一种Escherichia coli-Saccharomyces cerevisiae共同培养系统. 开发一个Escherichia coli-Saccharomyces cerevisiae共同培养系统.

主要成果:

工程化埃舍里希亚大肠杆菌产生了8毫克/升的二-β-离子子与前体积累.
麦芽菌 (Saccharomyces cerevisiae) 的转化效率更高,将β-离子子转化为二-β-离子.
共同培养系统最终实现了27 mg/L的二-β-离子子子位.

结论:

开发了一个有效的微生物平台,用于生物基二-β-离子子生产.
共同培养工程是一种可行的策略,用于增强扩展代谢途径中的标位.
这项研究为可持续生产有价值的风味和香味化合物提供了基础.

关键词:

生物合成生物合成共同培养是一种共同文化.这是一种二-β-ionone.代谢工程是代谢工程.自然的风味和香味.

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