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多种策略,包括6mA甲基化,影响植物替代拼接在全聚类花生

Lihui Wang¹, Hua Chen², Yuhui Zhuang³

¹Center for Legume Plant Genetics and System Biology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

Plant biotechnology journal

|January 31, 2024

概括

这项研究揭示了花生中的替代拼接 (AS),增强了基因调节和基因组注释. 染色体结构,6mA和可转移元素显著影响AS和基因表达.

科学领域:

基因组学和分子生物学
植物科学植物科学
转录后条例转录后条例转录后条例

背景情况:

替代拼接 (AS) 增加了真核生物的转录多样性,但其在植物中的综合调节仍未得到充分研究.
花生 (Arachis hypogaea) 的基因表达和调节需要更深入的研究.

研究的目的:

综合分析花生中使用多omics方法的替代拼接事件.
改进花生基因组注释,了解植物AS.的调控机制.

主要方法:

长读异形序列化 (Iso-Seq) 来识别全长的转录和拼接接口.
聚亚脱位的表征,包括替代性聚亚脱 (APA).
综合色素结构,6mA修饰和可转移元素 (TE) 数据的多omics分析.

主要成果:

获得了146,464个全长的转录,纠正了1,782个基因注释,并确定了4,653个新的位置.
鉴定了50977个多亚脱位,其中12369个呈现出替代多亚脱位.
特定染色体区域 (TAD边界,端粒,循环) 的基因显示出更多的独特异构形,更高的表达,更低的6mA,以及更多的TE.
观察到6mA对AS和基因表达的抑制作用.
确定了长非编码RNA和融合转录.

结论:

多omics分析提供了精细的花生基因组注释,并加深了对AS调节的理解.
染色体相互作用,6mA和TE是调节花生中AS和基因表达的关键因素.
替代拼接与植物中的多种基因调节策略有关.

关键词:

在6mA的6mA.这就是Iso-Seq.另一个替代拼接方法是拼接.染色体结构染色体结构花生花生花生花生花生

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