开发一种在线同位素稀释CE/ICP-MS方法,用于生物化合物中硫的量化
Dariya Tukhmetova1, Jan Lisec2, Jochen Vogl1
1Federal Institute for Materials Research and Testing (BAM), Division 1.1─Inorganic Trace Analysis, Richard-Willstätter-Str. 11, 12489 Berlin, Germany.
Analytical chemistry
|January 31, 2024
概括
一种新方法使用毛细管电泳和ICP质谱测量量生物分子中的硫. 这项技术准确地测量了像血清这样复杂样本中的蛋白质和其他生物分子.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 准确量化生物分子中硫的含量对于各种科学领域至关重要.
- 现有的方法可能会面临复杂的矩阵和蛋白质吸附的挑战.
- 不特定物种对硫的量化提供了一种多用途的方法.
研究的目的:
- 开发和验证用于定量生物分子中的硫的分析方法.
- 为了能够准确地确定蛋白质和其他生物分子在复杂的生物矩阵.
- 在效率和准确性方面改进现有的硫量化方法.
主要方法:
- 在线同位素稀释 (ID) 与毛细电泳 (CE) 和感应合等离子体质谱 (ICP-MS) 相结合.
- 优化使用标准化合物 (硫酸盐,氨酸,氨酸,氨酸,氨酸,氨酸) 进行回收和检测极限.
- 在原生基质 (血清) 中验证,并应用于真实生物样本 (牛和人血清).
主要成果:
- 复合复原率在98%至105%之间,量化极限在4.1-8.4毫克L-1之间.
- 观察到高相关性 (r=0.99) 硫量化在5-100毫克L-1范围内.
- 精确量化血清中的白蛋白,相对扩展不确定性为6.7% (k=2).
结论:
- 在线开发的ID CE/ICP-MS方法为生物分子中的硫量化提供了准确可靠的方法.
- 该方法表现出更好的性能,包括减少蛋白质吸附和有效分离.
- 这种技术适用于各种领域,包括环境,生物,制药研究和临床诊断.
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