在大鼠的有氧运动期间调节肌肉内甘油三储量的GPR81的作用
Yihan Ni1, Xiangdeng Lai1, Lin Li1
11Institute of Sports Science, Sichuan University, Chengdu, China.
Physiology international
|January 31, 2024
概括
乳酸盐是一种运动代谢物,通过GPR81.1.影响脂质储存. 运动前的3-基酸盐 (3-OBA) 增强了信号传递,促进了脂肪分解,并增加了大鼠肌肉中的脂肪合成蛋白质表达.
科学领域:
- 运动生理学 运动生理学
- 分子生物学分子生物学
- 代谢调节 代谢调节 代谢调节
背景情况:
- 乳酸是一种关键的运动代谢物.
- G蛋白结合受体81 (GPR81) 是一种乳酸受体.
- 骨肌肉中的脂质代谢对于能量恒温至关重要.
研究的目的:
- 研究GPR81在调节大鼠胃角肌肉中的脂质储存中的作用.
- 确定3-基酸盐 (3-OBA) 对运动期间cAMP-PKA通路和脂解的影响.
主要方法:
- 在老鼠体育炼前15分钟服用3-OBA (3毫摩尔L-1).
- 测量了cAMP-PKA信号通路的活动.
- 评估脂解 (HSL,ATGL) 和脂生 (ACC,FAS) 相关酶的蛋白质表达.
- 量化线粒体标志物 (COX IV,CS活性).
主要成果:
- 运动增加了肌肉内乳酸.
- 3-OBA预处理增强了cAMP-PKA信号传输.
- 3-OBA增加了脂解蛋白 (HSL,ATGL) 的表达.
- 3-OBA降低了脂质生成蛋白 (ACC,FAS) 的表达.
- 线粒体标记物 (COX IV,CS) 增加,但肌肉内甘油三酸 (IMTG) 含量保持不变.
结论:
- 乳酸,可能通过GPR81,在运动期间影响骨肌肉中的脂解和脂生成.
- 3-OBA的使用调节了这些通路,这表明它在能量基板利用中发挥了作用.
- 需要进一步的研究,以充分阐明对IMTG内容的影响.
相关概念视频
01:06Fats as Energy Storage Molecules
25.2K
Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
25.2K
01:09GPCRs Regulate Adenylyl Cylase Activity
5.6K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.6K