脂肪酶衍生干细胞外体促进瘤特征和免疫抑制微环境在乳腺癌
Qin Zhu1, Yukun Cao1, Jiaqi Yuan1
1Department of General Surgery, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, 410008, Hunan, People's Republic of China.
Cancer immunology, immunotherapy : CII
|January 31, 2024
概括
脂肪酸衍生干细胞外体 (ASC-exos) 促进乳腺癌的生长,并通过改变T细胞和巨细胞的分化,创造一个免疫抑制瘤微环境. 这突出了乳腺癌治疗的潜在治疗目标.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
背景情况:
- 脂肪衍生干细胞 (ASC) 和自身脂肪移植正在探索乳腺癌的重建.
- 了解ASC外体 (ASC外体) 在乳腺癌进展和瘤微环境中的作用至关重要.
研究的目的:
- 研究ASC和ASC外体对乳腺癌特征和瘤微环境免疫的影响.
- 探索ASC-exos在调节乳腺癌进展中的潜在应用.
主要方法:
- 从人类脂肪组织中分离和验证ASC外体.
- 与CD4+ T细胞,CD14+单细胞和MCF-7乳腺癌细胞一起培养ASC-exos.
- 在裸体小鼠的体内瘤形成研究.
- 细胞特征测试 (CCK8,scratch,Transwell),组织病理学 (HE,IHC,IF),流细胞测量,西斑,qRT-PCR和RNA测序.
主要成果:
- ASC-exos促进了迁移,增殖,入侵和瘤形成,同时抑制了MCF-7细胞的亡.
- ASC-exos抑制了Th1/Th17分化,并促进了CD4+T细胞中的Treg分化.
- ASC-exos抑制了M1分化,并促进了CD14+单细胞中的M2分化,导致瘤微环境免疫抑制.
- 在ASC-exos治疗组中观察到TOX,CD4,LYZ1的上调和Mettl7b,Serpinb2基因的下调.
结论:
- ASC-exos促进乳腺癌的进展,并通过调节T细胞和巨细胞分化,创造一个免疫抑制瘤微环境.
- ASC-exos是调节乳腺癌免疫力和进展的潜在治疗标.
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