检测了六个基因中的多态性,并分析了它们与绵羊子大小的关联
Kai Liu1, Yufang Liu1, Mingxing Chu1
1State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
Animal biotechnology
|January 31, 2024
概括
绵羊中的遗传标记物,特别是CLSTN2和CTH基因,与 litter 规模的增加有关. 这项研究确定了提高绵羊生殖效率的潜在遗传工具.
科学领域:
- 动物遗传学动物遗传学
- 生殖生物学 生殖生物学
- 定量性质遗传学 定量性质遗传学
背景情况:
- 羊的垃圾大小是一个复杂的特征,受多个基因的影响.
- 一些基因,包括APAF1,CLSTN2,CTH,PLCB1,PLCB4和CHST11,都与哺乳动物的繁殖有关.
- 这些基因对绵羊子大小的特定影响仍然在很大程度上未被描述.
研究的目的:
- 研究六个候选基因中的单核酸多态 (SNPs) 与绵羊的 litter 尺寸之间的关联.
- 在五种绵羊品种中分析这些SNP的种群遗传多样性和哈迪-韦恩伯格平衡.
- 识别特定的遗传标记,可用于提高绵羊的子大小.
主要方法:
- 在六个生殖基因 (APAF1,CLSTN2,CTH,PLCB1,PLCB4,CHST11) 中使用Sequenom MassARRAY® SNP测定技术对SNP进行基因定型.
- 种群遗传分析,包括多态信息内容和哈迪-韦恩伯格平衡测试.
- 在小尾汉羊中,确定了SNP与幼大小之间的关联分析,用于第一和第二平价.
- 对CLSTN2和CTH的蛋白相互作用网络的预测.
主要成果:
- 大多数绵羊品种每位表现出三种基因型,多态信息含量从0到0.37.
- 人口遗传分析表明,大多数品种处于哈迪-韦恩伯格平衡状态 (p > 0.05).
- 在CLSTN2的rs399534524位点与第一个平价 litter 尺寸显著相关 (CT基因型显示较高的 litter 尺寸).
- 在CTH的rs407142552位点与第二平价 litter 尺寸显著相关 (CT基因型显示 litter 尺寸更高).
- 蛋白相互作用网络分析揭示了CLSTN2与HTR1E,NOM1,CCDC174和ALPK3的潜在相互作用,这些相互作用也与 litter 尺寸有关.
结论:
- 在CLSTN2和CTH基因中的特定SNP与小尾汉羊的子大小变化有关.
- 这些已识别的基因位置可以作为有价值的分子标记,用于标记器辅助的选择,以改善绵羊子大小.
- 对CLSTN2和CTH的蛋白相互作用网络的进一步调查可能会揭示影响绵羊生殖特征的其他遗传因素.
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