探索参与结肠癌进展的关键基因和机制,基于生物信息学分析
Yongting Lan1, Xiuzhen Yang2, Yulian Wei3
1Department of Gastroenterology, Zibo Central Hospital, Zibo, 255036, Shandong, China.
Applied biochemistry and biotechnology
|January 31, 2024
概括
这项研究确定了CCNB1,CLCA1和PLK4作为抑制结肠癌进展的关键基因,通过减少癌细胞增殖和迁移,同时促进细胞亡. 这些发现为结肠癌治疗提供了潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 结肠癌的进展涉及复杂的分子机制.
- 确定预后生物标志物对于患者的治疗结果至关重要.
研究的目的:
- 为了探索结肠癌进展机制.
- 为了确定与结肠癌患者预后相关的枢纽基因.
主要方法:
- 利用基因表达综合 (GEO) 数据集 (GSE10950,GSE62932) 和 GEO2R 来识别差异表达基因 (DEG).
- 进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
- 使用STRING和Cytoscape识别枢纽基因的构建蛋白质-蛋白质相互作用 (PPI) 网络.
- 使用癌症基因组图谱 (TCGA) 数据和卡普兰-梅尔生存分析验证了与预后相关的枢纽基因.
- 经过实验验证的基因表达和功能作用在结肠癌细胞系 (LOVO) 和正常细胞 (NCM-460) 中,通过RT-qPCR,西斑和功能分析 (增殖,迁移,亡) 进行实验验证.
主要成果:
- 确定了266种常见的DEGs,在细胞循环和代谢途径中富含.
- 发现了10个枢纽基因,其中CCNB1,CLCA1和PLK4被确定与预后有显著关联.
- 证明增加CCNB1,CLCA1和PLK4的表达抑制了结肠癌细胞的增殖和迁移,同时诱导了亡.
结论:
- CCNB1,CLCA1和PLK4被确定为可以抑制结肠癌进展的关键基因.
- 这些基因有可能成为结肠癌治疗的治疗标.
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