色素向可见光诱导的光-CORM用于同时释放CO和单一氧气生成
Upendar Reddy Gandra1,2, Batakrishna Jana3, Patrick Hammer4
1Inorganic and Analytical Chemistry (IAAC), Friedrich Schiller University Jena, Humboldtstr. 8, D-07743 Jena, Germany. upreddygandra@gmail.com.
概括
我们开发了一种稳定,光激活的分子 (Lyso-CORM),可以在 lysosomes 中释放一氧化碳 (CO) 和单片氧 (O2) . 这显示了光疗的希望,因为它的向作用和最小的黑暗毒性.
科学领域:
- 生物医学工程 生物医学工程
- 光动力学疗法 光动力学疗法
- 化学生物学 化学生物学
背景情况:
- 碳一氧化物释放分子 (CORM) 正在探索治疗应用.
- 光动力疗法 (PDT) 利用光产生反应性氧物种进行治疗.
- 向特定的细胞器官,如溶解体,可以提高治疗疗效,减少副作用.
研究的目的:
- 开发和描述一种针对溶酶体的新型,对光敏感的一氧化碳释放分子 (Lyso-CORM).
- 在光激发后研究Lyso-CORM的CO和单片氧 (1O2) 生成能力.
- 为了评估Lyso-CORM在潜在的光疗应用中的光毒性,生物相容性和溶酶体向性.
主要方法:
- 莱索-CORM的合成和表征.
- 光物理研究以评估在一光和两光子激发 (800 nm) 下的CO和O2生成.
- 细胞毒性测定用于在相关细胞模型中确定光毒性和暗毒性.
- 细胞成像以确认 lysosome 特定的向.
主要成果:
- 在黑暗条件下,Lyso-CORM表现出高稳定性.
- 通过800nm光辐射 (一光和两光子) 实现了有效的CO和O2生成.
- 莱索-CORM表现出显著的光毒性,归因于CO和O2的协同效应,黑暗毒性微不足道,具有特定的溶酶体积累.
结论:
- 莱索-CORM是一种稳定,化体向的,光性分子,能够释放CO和O2.
- 在光照射下CO和O2的联合作用导致强烈的光毒性.
- 莱索-CORM的特定向,生物相容性和光动力学特性使其成为先进光疗法策略的有希望的候选人.
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