下垂体-垂体-卵巢/甲状腺轴的分子机制调节的第一个蛋时的年龄
Zhiyu He1, Qingyuan Ouyang1, Qingliang Chen1
1Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China.
Poultry science
|January 31, 2024
概括
这项研究揭示了下丘脑 - 下垂体 - 卵巢 (HPO) 和下丘脑 - 下垂体 - 甲状腺 (HPT) 轴如何相互作用,以控制的第一个蛋的年龄. 像GREB1和OPN5这样的关键基因被认为对这种共同调节至关重要.
科学领域:
- 禽畜科学 禽畜科学 禽畜科学
- 内分泌学 在内分泌学.
- 遗传学 是一个遗传学.
背景情况:
- 第一次蛋的年龄 (AFE) 是家禽的关键生殖特征.
- 下垂体 - 下垂体 - 卵巢 (HPO) 和下垂体 - 下垂体 - 甲状腺 (HPT) 轴是已知的性成熟的调节者.
- 有证据表明,HPO和HPT轴之间存在共同调节关系,但AFE的潜在机制仍未被探索.
研究的目的:
- 研究HPO和HPT轴之间的交叉声在中调节AFE时的交叉声.
- 确定参与AFE联合调节的关键途径和基因.
主要方法:
- 在正常和异常产卵之间对甲状腺和卵巢指数进行比较形态分析.
- 对下丘脑,垂体,甲状腺和卵巢的转录分析.
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
- 蛋白与蛋白相互作用 (PPI) 网络和相关性分析.
主要成果:
- 在正常和异常产卵组之间观察到甲状腺和卵巢指数的显著差异.
- 在四种组织中发现了许多差异表达基因 (DEG).
- 在所有组织中,MAPK信号通路得到了一致的丰富.
- 确定GREB1和OPN5是HPO和HPT轴之间的交叉声调节的关键调节者,分别影响雌激素和甲状腺激素信号传递.
- 他们确定了10个候选基因,包括IGF1,JUN,ERBB4,KDR,PGF,FGFR1,GREB1,OPN5,DIO3和THRB.
结论:
- HPO和HPT轴在AFE的调节中表现出显著的交叉声.
- GREB1和OPN5在调解这种相互作用方面发挥着关键作用.
- 已识别的候选基因为了解家禽中AFE的遗传和生理机制提供了基础.
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