精子暴露于辅助腺体分泌物会改变牛体内膜的转录基因反应
José María Sánchez1, María Belén Rabaglino2, Sandra Bagés-Arnal2
1Department of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), Spanish National Research Council (CSIC), Madrid, Spain.
Theriogenology
|January 31, 2024
概括
辅助腺体的分泌物影响牛的精子-子宫内膜相互作用. 排泄的精子暴露在这些分泌物中,诱导的转录变化比 epididymal 精子少,表明辅助腺因子调节子宫反应.
科学领域:
- 生殖生物学 生殖生物学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 精子在交配后影响牛子宫内膜转录组.
- 在这种调制中,辅助腺体 (AG) 因素与内在精子因素的作用尚不清楚.
研究的目的:
- 为了研究排精精子 (暴露于AG分泌物) 或皮膜精子 (未暴露) 是否引起牛体内膜异位转录基因反应的不同.
主要方法:
- 来自雌性公牛的牛子宫内膜外置被与 epididymal 精子或射精精子共化 6 小时.
- 用RNA测序分析子宫内膜的转录组变化.
主要成果:
- 流行性精子诱导的差异性表达基因 (DEGs) 的数量 (1912) 与排泄精子相比 (115).
- 流行性精子上调调节了与T细胞调节和TNF,NF-KB和IL17信号传递相关的通路.
- 排泄精子调节了参与T细胞免疫和NF-KB信号传递的基因,与皮膜精子不同.
结论:
- 辅助腺因子显著调节精子与牛子宫内膜之间的相互作用.
- 这些发现突出了辅助腺分泌在调节生殖期间子宫环境中的重要性.
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