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影响冷/解马精液精子质量分析和解释的因素

Camilo Hernández-Avilés¹, Luisa Ramírez-Agámez¹, Dickson D Varner¹

¹Department of Large Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4475, USA.

|January 31, 2024

概括

核子计数器和流动细胞计量显示,对马精子生存能力的高度一致. 精子运动性和活力协议根据结前的质量而异,脂质过氧化不能完全解释差异. 精子质量分析是可重复的,不受解后稀释的影响.

科学领域:

动物科学动物科学
生殖生物学生殖生物学
兽医医学兽医医学兽医医学

背景情况:

精子活力和运动性的准确评估对于马的生育能力至关重要.
制冷保存和解后分析方法的标准化对于马类繁殖至关重要.

研究的目的:

为了评估NucleoCounter-SP100和流细胞计之间的协议,以确定马精子的生存能力.
评估经解后的精子运动性和活力之间的相关性.
为了研究脂质过氧化在活力-运动性差异中的作用.
为了确定精子质量分析的可重复性和解后稀释对运动性的影响.

主要方法:

与NucleoCounter-SP100 (NC) 和流细胞计 (FC) 进行精子活力 (VIAB) 的比较.
评估总运动性 (TMOT) 和VIAB协议,包括分析脂质过氧化 (VLPP).
评估经解后的精子质量分析可重复性和精液稀释的影响 (10,30,50 x 10^6精子/毫升).

主要成果:

在NC和FC之间达成高度一致 (偏差:1到-3) 和类似的解后VIAB.
在TMOT和VIAB之间减少一致性 (偏见:-4到-25) 与较低的冷前精子形态和DNA质量相关.
在VIAB - TMOT和VLPP之间的偏差在5到7之间.
根据分析的重复性或稀释率,在解后的精子质量或运动特征中没有发现显著差异.

结论:

核子计数器-SP100是流动细胞计量的可靠替代品,用于评估马精子的生存能力.
除了脂质过氧化之外的因素影响了精子运动性和活力之间的关系.
在马匹中,解后的精子质量评估是可重复的,不受常见稀释率的影响.

关键词:

协议协议协议协议冰/解的精液是可以使用的.移动性移动性移动性马匹的精子是马匹的精子.可行性可行性.

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