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对诱导铁缺乏耐受性的相互主义的分子洞察力,在注射Trichoderma harzianum的中诱导铁缺乏耐受性

Ahmad H Kabir¹, Jeffrey L Bennetzen²

¹School of Sciences, University of Louisiana at Monroe, LA 71209, USA; Department of Genetics, University of Georgia, Athens, GA 30602, USA.

Microbiological research

|January 31, 2024

概括

土壤真菌Trichoderma harzianum通过增强根植殖和铁运输基因表达来显著改善缺铁的铁吸收和植物健康. 这项研究揭示了T. harzianum背后的机制.

科学领域:

植物科学植物科学
土壤微生物学土壤微生物学
生物化学生物化学

背景情况:

缺铁是一种主要的非生物压力,限制了作物的生产力,特别是在高pH的土壤中种植的中.
众所周知,有益的土壤真菌Trichoderma harzianum可以缓解铁缺乏症,但其确切的机制尚不清楚.

研究的目的:

阐明Trichoderma harzianum减轻中铁缺乏症的分子和生理机制.
调查根植入,基因表达和生化变化在铁应力下的-T. harzianum相互作用中的作用.

主要方法:

是在高pH诱导的铁缺乏症下在中种植的,有或没有T. harzianum注射.
评估了形态生理特征,铁的吸收和氧化还原状态.
进行了根尖和真菌殖民 (ThAOX1基因) 的基因表达分析 (RNA-seq,qPCR);量化了 siderophore 和 indole 乙酸 (IAA) 的水平.

主要成果:

T. harzianum殖民显著改善了的生物质,光合作用,用水效率以及根和芽中的铁含量.
在根中观察到参与铁吸收 (SbTOM2,SbYS1),牛津合成 (SbSAURX15) 和尼古丁胺合成酶 (SbNAS3) 的关键基因的升调.
T. harzianum诱导其 siderophore 合成基因 (ThSIT1),导致 siderophore 和 IAA 生产增加,促进铁化和吸收.

结论:

T. harzianum通过产生 siderophores 和诱导植物铁吸收载体 (如SbYS1.1) 来提高的铁可用性.
这种真菌还通过过氧化酶和ABA基因诱导刺激植物的适应反应,包括辅酶合成和对氧化应激的耐受性.
这项研究为T. harzianum在对抗中铁缺乏症的有效性提供了第一个机制性的洞察力,支持其用于高pH环境的生物肥料中的使用.

关键词:

辅助剂辅助剂辅助剂辅助剂生物肥料生物肥料生物肥料这些真菌是 siderophore 菌类.战略II Fe吸收情况这种共生是共生.

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