相关实验视频

Updated: Jul 4, 2025

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

加斯德明D调节了巨细胞中可溶性fms类铁酶-1的释放

Hazuki Tanaka¹, Ren Ozawa¹, Yuka Henmi¹

¹Laboratory of Animal Reproduction, Department of Animal Science, Tokyo University of Agriculture, Japan.

Reproductive biology

|January 31, 2024

概括

人类巨细胞通过气体皮质蛋白D (GSDMD) 通过可溶性fms类型的氨酸激酶 (sFLT1) 分泌,这是一种涉及孕前 (PE) 病理学的途径. 这种sFLT1释放与cathepsin B有关,而不是NLRP3炎症酶激活.

科学领域:

免疫学免疫学免疫学
病理学病理学病理学
细胞生物学细胞生物学

背景情况:

孕前 (PE) 是一种严重的妊娠并发症,与胎盘可溶性fms类氨酸激酶 (sFLT1) 有关.
虽然热囊细胞是主要来源,但胎盘单细胞/巨细胞也分泌sFLT1.1.
NLRP3炎症酶和气皮素D (GSDMD) 调节的热与PE的发病有关.

研究的目的:

调查人类单细胞/巨细胞THP-1细胞是否分泌依赖NLRP3炎症体和GSDMD的sFLT1.
探索GSDMD和cathepsin B在sFLT1分泌中的作用.

主要方法:

使用博12-米里酸13-酸盐 (PMA) 将THP-1单细胞分化为巨细胞.
使用了NLRP3,caspase-1和GSDMD的基因删除和药理抑制剂.
评估sFLT1和IL-1β分泌和细胞死亡.
通过使用CA074-ME.ME研究了cathepsin B的作用.

主要成果:

在THP-1巨细胞中,PMA诱导了sFLT1和IL-1β的分泌,但没有导致细胞死亡.
NLRP3炎症酶组件 (NLRP3,卡斯帕酶-1) 对sFLT1分泌没有必要.
删除或抑制GSDMD显著降低了IL-1β和sFLT1分泌.
素B的抑制也降低了sFLT1和IL-1β的释放.

结论:

THP-1巨细胞以GSDMD依赖的方式释放sFLT1,但不是NLRP3炎症酶依赖的方式.
这种由GSDMD介导的sFLT1释放可能涉及GSDMD的非性功能.
sFLT1分泌与PMA刺激的巨细胞中的lysosomal cathepsin B活性有关,这表明PE病理学中的作用.

关键词:

炎症炎症是一种炎症.lysosome 是一个溶解体.巨细胞是一个巨细胞.孕前症孕前症热性灭症 (Pyroptosis) 是一种

更多相关视频

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

Published on: May 24, 2019

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Amplifying Signals via Enzymatic Cascade

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...