相关实验视频

Updated: Jul 4, 2025

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

由代谢工程Pseudomonas putida同时利用葡萄糖和氧化糖生产3-基酸

Rameshwar Tiwari¹, Chandran Sathesh-Prabu¹, Yuchan Kim¹

¹School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea.

Bioresource technology

|January 31, 2024

概括

工程造型的Pseudomonas putida通过韦姆伯格途径有效地代谢氧化糖. 适应性进化和基因改造提高了西洛斯的利用率,使生物炼油厂可以从生物质水解物中产生强大的3-基酸.

科学领域:

代谢工程是代谢工程.
合成生物学合成生物学
工业生物技术工业生物技术

背景情况:

伪虫 (Pseudomonas putida) 是一个关键的微生物,用于在生物炼油厂中利用纤维素生物质.
氧化糖的高效代谢,一个主要的构成部分的纤维素生物质,仍然是一个挑战的P. putida.
引入韦姆伯格通路是一种使得克西洛斯代谢成为可能的策略.

研究的目的:

为了改造Pseudomonas putida,以提高草甘的利用率.
开发一个微生物细胞工厂,从纤维素糖中生产3-基酸 (3HP).
评估工程菌株在利用生物质水解剂中的性能.

主要方法:

引入韦姆伯格通路到P. putida EM42在一个酶诱导促进剂下.
适应性实验室进化 (ALE) 改善西洛斯代谢.
全基因组测序以确定有益的突变.
将关键突变逆向工程化为父株.
为了生产3基酸 (3HP) 的工程工程应变.

主要成果:

最初的工程菌株在西洛斯 (0.05h-1) 上显示缓慢增长.
ALE导致了一种具有显著增强西洛斯生长 (0.36小时−1) 的菌株,与葡萄糖生长 (0.39小时−1) 相似.
在PP3380和PP2219中发现并验证了关键突变.
反向工程的菌株有效地共用葡萄糖和西洛斯 (0.3 g/L/h).
改造品种从葡萄糖和树脂糖中产生了29g/L的3HP.
使用空棕果束水解剂实现了有前途的3HP生产.

结论:

适应性实验室进化和有针对性的基因工程可以克服Pseudomonas putida中西洛斯代谢的挑战.
改造的P. putida菌株显示出作为一种细胞工厂的巨大潜力,可以从可再生资源中生产3HP等有价值的化学品.
这项工作有助于开发可持续的生物炼油厂,利用基纤维素生物质.

关键词:

3-基烯酸是3基烯酸中的一种.适应性实验室进化的演变.伪omonas putida可以使用.姆堡路径的路径.克西洛斯的新陈代谢

更多相关视频

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

相关概念视频

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

Glycolysis: Preparatory Phase

Glycolysis: Preparatory Phase

In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...

Energy-requiring Steps of Glycolysis

Energy-requiring Steps of Glycolysis

Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...