一个jingmenvirusRNA依赖RNA聚合酶在结构上类似于flavivirus对应物,但在启动阶段具有不同的特征
Xinyu Wang1,2, Xuping Jing1, Junming Shi2,3
1Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, No. 262 Jin Long Street, Wuhan, Hubei 430207, China.
Nucleic acids research
|January 31, 2024
概括
京门病毒NSP1 RdRP与病毒NS5 RdRP具有结构上的相似性. 这项研究揭示了JMTV RdRP独特的酶性质和潜在的MTase依赖性,澄清了jingmenvirus-flavivirus进化联系.
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- 京门病毒是新兴的细分病毒,与病毒有关.
- 病毒非结构蛋白1 (NSP1) 含有甲基转移酶 (MTase) 和依赖RNA的RNA聚合酶 (RdRP) 域,对复制至关重要.
- 京门病毒 (JMTV) 是京门病毒的类型物种.
研究的目的:
- 为了确定JMTV NSP1 RdRP模块的晶体结构.
- 描述JMTV NSP1 RdRP. 的酶性质.
- 将JMTV RdRP与其他Flaviviridae RdRP进行比较,并阐明其与MTase域的功能关系.
主要方法:
- 通过X射线晶体学来解决JMTV NSP1 RdRP.的1.8 Å分辨率结构.
- 酶检测分析RdRP活性,包括堕胎产品形成,启动偏好和原料使用.
- 与代表性的Flaviviridae RdRP进行比较分析.
主要成果:
- 尽管低序列相同,但JMTV NSP1 RdRP结构与flavivirus NS5 RdRP非常相似.
- JMTV NSP1 RdRP生产三倍流产产品,并且更喜欢使用短启动器进行终端启动.
- 一个没有MTase的JMTV NSP1结构显示了产品分布的改变,这表明MTase参与了延长过渡.
结论:
- 这项研究证实了人病毒和Flaviviridae家族之间的进化关系.
- 与flavivirus NS5.5相比,JMTV NSP1 RdRP具有独特的酶特性.
- 这些发现提供了对jingmen病毒的复制和转录机制的见解.
相关概念视频
Retrovirus Life Cycles
46.0K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
46.0K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Eukaryotic RNA Polymerases
24.2K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.2K
Viral Structure
62.3K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.3K
Initiation of Translation
32.9K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
32.9K
Bacterial RNA Polymerase
29.5K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.5K


