尿液生物标志物单独和作为共识模型显示高灵敏度和特异性检测尿路感染
Marzieh Akhlaghpour1, Emery Haley2, Laura Parnell3
1Department of Research and Development, Pathnostics, 15545 Sand Canyon Suite 100, Irvine, CA, 92618, USA.
BMC infectious diseases
|January 31, 2024
概括
新的尿路生物标志物可以准确地识别尿路感染 (UTI). 中性粒细胞凝酶相关的利波卡林 (NGAL) 和白内素 (IL-8,IL-1β) 在诊断尿路感染方面具有很高的准确性,特别是当标准测试没有确定性时.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 传染性疾病 传染性疾病
- 生物标志物发现发现
背景情况:
- 用于诊断尿路感染 (UTI) 的标准尿培养 (SUC) 在敏感性和识别多微生物感染方面存在局限性.
- 高比例的SUC阴性结果和无症状细菌尿需要准确的诊断方法.
- 这项研究研究了与感染相关的尿路生物标志物,以区分真正的尿路感染病例与非尿路感染的对照.
研究的目的:
- 评估尿路生物标志物的有效性,以区分确定的尿路感染病例与非尿路感染的对照病例.
- 评估NGAL,IL-8和IL-1β在尿路感染诊断中的诊断性能.
- 确定这些生物标志物的共识是否可以提高尿路感染诊断的准确性.
主要方法:
- 从有症状的尿路感染患者和无症状的志愿者收集尿样.
- 使用标准尿液培养 (SUC) 和多重PCR (M-PCR) 进行了微生物分析.
- 尿路生物标志物NGAL,IL-8和IL-1β使用ELISA进行测量.
主要成果:
- 在微生物密度和NGAL,IL-8和IL-1β水平之间发现了强烈的正相关性.
- 具有≥2个阳性生物标志物的共识标准在区分尿路感染病例时获得了84.0%的灵敏度和91.2%的特异性.
- 与无症状对照人群相比,症状性尿路感染病例中的生物标志物NGAL,IL-8和IL-1β显著升高.
结论:
- 阳性尿路生物标志物NGAL,IL-8和IL-1β与确定的尿路感染病例有关.
- 使用这些生物标志物的共识标准是活跃的尿路感染的优秀支持性诊断工具.
- 这种生物标志物方法在SUC和M-PCR结果不一致时尤为有价值.
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