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[脂质衍生干细胞衍生外体通过mTOR途径调节AR患者外周血液中的Th2/Treg平衡]

Feiyan Han¹, Xiaojie Xu¹, Ying Wang¹

¹Department of Otolaryngology Head and Neck Surgery，the First Affiliated Hospital of Zhengzhou University，Zhengzhou，450052，China.

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery

|February 1, 2024

概括

脂肪基衍生干细胞外体 (ADSC-exos) 显示出通过恢复Th2和调节性T (Treg) 细胞的平衡来治疗过敏性鼻炎 (AR) 的潜力. 这通过PI3K/AKT/mTOR/p70S6K信号通路发生,这表明了AR的新治疗方法.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

过敏性鼻炎 (AR) 的特征是T助手2 (Th2) 和调节性T (Treg) 细胞的不平衡.
PI3K/AKT/mTOR/p70S6K信号通路与免疫细胞的功能和分化有关.

研究的目的:

研究由脂肪衍生干细胞外体 (ADSC-exos) 调节AR患者Th2/Treg平衡的机制.
探索PI3K/AKT/mTOR/p70S6K路径在本规则中的作用.

主要方法:

来自AR患者和健康对照者的外周血液样本被分析为细胞因子水平 (IL-4,TGF-β) 和免疫细胞群 (Th2,Treg).
关键途径组件 (PI3K,AKT,mTOR,p70S6K) 的基因和蛋白质表达被使用qRT-PCR和Western Blotting测量.
来自AR患者的外周血液单核细胞 (PBMC) 用ADSC-exos治疗,并评估了途径激活和细胞平衡的随后变化.

主要成果:

与对照组相比,AR患者表现出激活PI3K/AKT/mTOR/p70S6K通路,增加IL-4,降低TGF-β,Th2细胞比例较高,Treg细胞比例较低.
在AR PBMC中ADSC-exos治疗导致mTOR通路激活减少,IL-4减少,TGF-β增加,Th2细胞减少和Treg细胞增加.

结论:

AR与Th2/Treg细胞失衡和PI3K/AKT/mTOR/p70S6K通路在PBMCs中的激活有关.
ADSC-exos显示出在AR中恢复Th2/Treg平衡的潜力,可能通过PI3K/AKT/mTOR/p70S6K通路的调节.

关键词:

在Th2细胞中.在Treg细胞中.脂肪衍生干细胞来源的干细胞.外基因组是外基因组的组成部分.在 mTOR 路径上.

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