氧沙维科尔通过抑制NF-κB信号通路来抑制体外骨质细胞形成
Sirada Srihirun1, Satarat Mathithiphark2, Chareerut Phruksaniyom1
1Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand.
Biomolecules & therapeutics
|February 1, 2024
概括
贝特尔树叶中的化合物Hydroxychavicol有效地抑制了骨质细胞形成,即骨分解的过程. 它抑制了参与骨重塑的关键途径,为骨损失提供了潜在的治疗益处.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 贝特尔树叶中含有的化合物Hydroxychavicol在此之前已经显示出骨质生成性.
- 基沙维科尔对骨质细胞介导的骨重塑的影响仍然没有被描述.
研究的目的:
- 为了研究氧沙维醇的抗骨质结晶效应.
- 阐明氧化对骨质细胞作用的潜在分子机制.
主要方法:
- 评估了Hydroxychavicol对来自RAW264.7细胞和人体外围单核细胞 (PBMCs) 的核因子卡帕-B连接体 (RANKL) 诱导骨质细胞的受体激活剂的影响.
- 分析了关键的骨质细胞特异性基因 (甲素K,MMP-9,DC-STAMP) 和转录因子 (NFATc1,c-Fos,c-Jun).
- 研究了NF-κB信号通路,包括p65和Iκβα酸化和p65核转位.
主要成果:
- 氧沙维科尔剂量依赖性降低了骨质细胞形成,F-actin环发育和骨再吸收活性.
- 骨质细胞特异性基因和关键转录因子 (NFATc1,c-Fos,c-Jun) 的表达得到了显著的降低.
- 基沙维科尔通过阻断p65和Iκβα酸化来抑制RANKL诱导的NF-κB信号,从而减少p65的核转位.
结论:
- 在实验室中,Hydroxychavicol表现出强烈的抗骨质细胞生成活性.
- 该机制涉及通过抑制NF-κB信号通路来抑制RANKL诱导的NFATc1表达.
- 氧沙维科尔显示出作为治疗性剂的潜力,用于涉及过度骨再吸收的疾病.
关键词:
骨的再吸收 骨的再吸收这是一种氧化 (hydroxychavicol).在 NF-κBB 中.骨质结晶体 骨质结晶体 是一个骨质疏松症是一种骨质疏松症.在RAW264.7中,我们使用了RAW264.7更多相关视频
07:51Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
Published on: March 18, 2019
6.0K
11:47A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
Published on: June 8, 2014
11.7K
相关概念视频
Osteoclasts in Bone Remodeling
2.9K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
2.9K
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
Role of Vitamins in Maintaining Bone Health
3.3K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.3K
Hormones and Bone Tissue
2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K
Bone Remodeling
38.3K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.3K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
