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Updated: Jul 4, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
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对于生物催化剂的天然转氨酶融合

Luba Prout1, Helen C Hailes2, John M Ward1

  • 1Department of Biochemical Engineering, University College London London WC1E 6BT UK j.ward@ucl.ac.uk.

RSC advances
|February 1, 2024
PubMed
概括

研究人员确定了一种天然的转氨酶融合酶PP_2782及其同类. 这些酶有效地从各种基质中合成氨基,显示出工业生物催化剂应用的巨大潜力.

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Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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