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查糖尿病病的候选诊断生物标志物

Xinying Huang^1,2,3, Hui Zhang^1,2,3, Jihong Liu⁴

¹Department of Clinical Laboratory, the First Affiliated Hospital of Kunming Medical University, Kunming, China.

Journal of clinical laboratory analysis

|February 1, 2024

概括

糖尿病病 (DKD) 的早期诊断至关重要. 这项研究确定了新的尿路生物标志物SERPINA4和GGT1,与尿液肌素相结合,用于改善2型糖尿病患者的DKD检测.

科学领域:

腎臟病學 (nephrology) 是一種醫學專業.
蛋白质组学是指蛋白质组学.
生物标志物发现发现

背景情况:

糖尿病病 (DKD) 和非糖尿病病 (NDRD) 有不同的预后和治疗方法.
由于缺乏特定的生物标志物,早期诊断DKD受到阻碍.
尿液提供了一个非侵入性的来源,用于识别DKD生物标志物.

研究的目的:

使用蛋白质学识别糖尿病病 (DKD) 特定的新型尿路生物标志物.
通过尿蛋白分析,区分DKD与非糖尿病病 (NDRD).

主要方法:

无标签的定量蛋白质组学被用来分析来自DKD和NDRD患者的尿蛋白.
候选生物标志物使用并行反应监测 (PRM) 和酶相关免疫吸收试验 (ELISA) 进行了验证.
诊断的准确性是使用接收器操作特征曲线 (AUC) 下的面积来评估的.

主要成果:

在DKD和NDRD组之间确定了417种不同表达的.
三种蛋白质 (C7,SERPINA4,GGT1) 被验证为潜在的DKD生物标志物.
综合的SERPINA4/Ucr和GGT1/Ucr指数显示出显著的诊断效率 (AUC=0.758).

结论:

谢尔皮纳4 / Ucr和GGT1 / Ucr的综合指数显示,作为糖尿病病的诊断工具具有前途.
通过尿液肌素调整生物标志物水平可以提高诊断准确度.
这些发现有助于开发DKD的非侵入性诊断策略.

关键词:

生物标志物生物标志物脏病是一种神经病.蛋白质组学蛋白质组学尿道尿道尿道尿道

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