在乳制品乳清上种植复合的Aspergillus niger菌株作为碳水化合物来源
Teagan C Crament1, Kayline Arendsen1, Shaunita H Rose1
1Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa.
Journal of industrial microbiology & biotechnology
|February 1, 2024
概括
这项研究表明,基因工程Aspergillus niger可以利用乳制品废物 (乳清) 产生有价值的酶. 这表明了废物回收和负责任的商品生产的可持续方法.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 酵素工程是什么? 酶工程是什么
背景情况:
- 农业废物回收利用提供可持续的废物管理解决方案.
- 利用废物流用于商品生产符合负责任的制造业实践.
- 乳制品废弃物,特别是乳清,是一个未充分利用的资源.
研究的目的:
- 为了评估使用乳糖和乳制品乳清作为碳来源的复合Aspergillus niger菌株的内葡萄糖酶生产.
- 确定乳制品废弃物作为工业酶制造可行的原料的潜力.
- 为了优化乳清基媒体以提高复合酶产量.
主要方法:
- 开发出表达真菌内葡萄糖酶 (Trichoderma reesei eg1, eg2; Aspergillus carneus aceg) 的复合性尼日利亚菌D15菌株.
- 在乳糖和乳清介质上培育重组菌株,以评估内葡萄糖酶活性.
- 优化乳清介质的成分,以获得最具生产力的菌株 (A. niger D15[eg2]PyrG).
主要成果:
- 重组A. niger菌株在乳糖上达到34-54U/mL的最大内葡萄糖酶活性,在乳清上达到22-27U/mL.
- A. niger D15[eg2]PyrG菌株在优化10%乳清介质上产生了46个单位/毫升,其中0.6%的NaNO3.
- 使用乳清作为原料,证实了 endoglucanase 的显著产量.
结论:
- 乳清可以有效地作为生产重组酶的原料.
- 这种方法有助于可持续的废物管理和负责任的工业流程.
- 需要进一步研究以了解和减轻酶活动中观察到的变异.
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