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双功能的谷氨酸化核酸与CRISPR/Cas12a系统相结合,用于Hg²⁺的确定

Ying Yu¹, Yuan Zhang², Xu Chen²

¹Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Mikrochimica acta

|February 1, 2024

概括

一种新的光方法使用磁珠和CRISPR/Cas12a来检测离子 (Hg2+). 这种方法为监测牛奶和茶等食品样本中的Hg2+污染提供了高灵敏度.

科学领域:

分析化学分析化学
生物技术是生物技术.
环境科学环境科学

背景情况:

离子 (Hg2+) 构成重大环境和健康风险.
对Hg²⁺的敏感和选择性检测方法对于监测食品安全至关重要.

研究的目的:

开发一种高度敏感和选择性的光方法来检测Hg²⁺.
在Hg²⁺检测中利用CRISPR/Cas12a系统进行信号放大.

主要方法:

使用氨基基酸修饰的碳素磁珠 (CMB@APBA) 来捕获Hg2+.
作为双功能的DNA链接器,采用了glioxal中的核酸 (gcDNA).
集成了CRISPR/Cas12a系统,用于在Hg2+结合时生成放大光信号.

主要成果:

该方法表现出高灵敏度,Hg2+的检测极限为2.72nM.
在最佳条件下建立了20至250nm的线性检测范围.
在实物食品样本 (牛奶和茶饮料) 中成功检测出Hg2+ .

结论:

提出的基于CMB@APBA和CRISPR/Cas12a的光方法为Hg²⁺检测提供了一个敏感和选择性的平台.
这种方法对于现场监测食品中的Hg2+污染非常重要.
开发的测定为确保食品安全和环境保护提供了一个有前途的工具.

关键词:

双功能链接器双功能链接器酸; 经过修改的磁珠.在CRISPR/Cas12a;光检测检测.格里奥克萨尔中的核酸在Hg2+上.

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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...