PPAR-gamma对牛胚胎的发育能力和 trofhectoderm 血统规范有影响
Maura S McGraw1, Sandeep K Rajput2, Bradford W Daigneault1
1Department of Animal Sciences, University of Florida, Gainesville, Florida, USA.
Reproduction (Cambridge, England)
|February 1, 2024
概括
过氧体增殖器激活受体玛 (PPARG) 影响早期的牛胚胎发育和胎盘基因表达. 药理学操纵,而不是胚胎淘汰,揭示了其复杂的角色,表明母体和胚胎的贡献.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 过氧体增殖器激活受体玛 (PPARG) 对于胎盘功能至关重要.
- 它在早期胚胎发育和胎盘起源中的作用尚不清楚.
研究的目的:
- 研究牛PPARG在植入前胚胎发育中的作用.
- 确定其对细胞系特征和胎盘基因表达的影响.
主要方法:
- 在植入前胚胎中的牛PPARG的功能性特征.
- 使用罗西格利塔 (Ros) 和GW9662 (GW) 的PPARG的药理刺激和对抗作用.
- 胚胎基因敲除PPARG,并分析胚胎细胞的发育和基因表达.
主要成果:
- 从第6天开始,PPARG在牛胚胎中得到表达.
- 药理学PPARG调节影响了胚胎细胞的发育和细胞数量.
- 罗西格利塔治疗改变了延迟胚胎中关键胎盘发育基因的表达.
- 胚胎PPARG淘汰会影响总细胞数量,但不会影响芽细胞形成.
结论:
- PPARG在牛植入前胚胎发育和细胞系形成中起着调控作用.
- 母体和胚胎的PPARG都对胚胎发育和胎盘基因调节作出贡献.
- 药理学反应表明复杂的PPARG参与超出胚胎表达.
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