相关实验视频

Updated: Jul 4, 2025

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

在海马体中,WDR23调解NRF2蛋白质稳定和细胞保护能力

Jiahui Liu¹, Chatrawee Duangjan¹, Ronald W Irwin¹

¹University of Southern California, Leonard Davis School of Gerontology, Los Angeles, CA 90089, USA.

Mechanisms of ageing and development

|February 1, 2024

概括

研究人员发现,在小鼠中去除WDR23可以改善大脑功能,并防止神经炎症. 这表明,准WDR23-NRF2通路可以治疗神经退行性疾病,如阿尔茨海默氏症和帕金森症.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
衰老研究研究衰老研究

背景情况:

神经退行性疾病,包括阿尔茨海默病和帕金森病,涉及慢性神经炎症和蛋白质聚合,导致神经元死亡.
蛋白质平衡,蛋白质平衡的调节,对神经元健康至关重要,并且在衰老和神经退行性疾病中经常受到损害.

研究的目的:

研究WDR23 (一种CUL4-DDB1基质受体) 在大脑衰老和神经保护中的作用.
探索针对神经退行性疾病治疗干预的WDR23-NRF2信号的潜力.

主要方法:

创建了一个全球WDR23淘汰赛 (Wdr23KO) 的小鼠模型.
在Wdr23KO和野生型小鼠中评估与年龄相关的海马体依赖行为.
分析了NRF2/NFE2L2蛋白,相关RNA转录和抗氧化剂防御蛋白的稳定.

主要成果:

Wdr23KO小鼠表现出与年龄相关的海马体依赖行为的显著变化.
失去WDR23导致NRF2/NFE2L2蛋白的稳定.
在Wdr23KO动物中观察到由NRF2调节的RNA转录的增加和抗氧化剂防御蛋白的水平升高.

结论:

通过WDR23介导的蛋白质静止在维持海马内细胞保护能力方面发挥着至关重要的作用.
在神经退行性疾病中,WDR23-NRF2信号通路代表了对抗神经炎症和神经元细胞死亡的有希望的治疗标.

关键词:

行为行为行为.在海马体内,海马体鼠标鼠标是一个鼠标.在NRF2 / NFE2l2l2l2l2l2l2l2l2 这就是WDR23的意思.

更多相关视频

Measurements of Physiological Stress Responses in C. Elegans

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Published on: October 6, 2017

相关概念视频

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Role of Hippocampus in Memory

Role of Hippocampus in Memory

The hippocampus, a critical brain structure, plays an essential role in memory processing, particularly in the formation and retrieval of memory. This small, seahorse-shaped region is located within the medial temporal lobe, with one hippocampus in each brain hemisphere. Experimental studies involving lesions in the hippocampi of rats have demonstrated significant impairments in tasks such as object recognition and maze navigation, indicating the hippocampus involvement in both recognition and...

The Unfolded Protein Response

The Unfolded Protein Response

The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...