草百氧化素5和6介导的自抑制了草reovirus的复制,并减轻了氧化应激
Qian Wang1, Xinyu Liang1, Hanyue Wang1
1State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Fish & shellfish immunology
|February 1, 2024
概括
草的过氧化素 (Prxs) CiPrx5和CiPrx6增强了细胞对抗氧化应激和病毒感染的防御能力. 这些抗氧化酶促进了自,以抑制草重复病毒 (GCRV) 复制,并减少活性氧物种 (ROS).
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- 过氧素 (Prxs) 是关键的抗氧化酶,保护细胞免受反应性氧物种 (ROS) 引起的氧化损伤.
- 了解特定的PRX在鱼类免疫力和应激反应中的作用,对于水产养殖和疾病管理至关重要.
研究的目的:
- 克隆和表征两种草的过氧化基因,即CiPrx5和CiPrx6.6.
- 研究CiPrx5和CiPrx6在抗氧化应激和病毒感染的细胞防御中的功能,特别是草重生病毒 (GCRV).
主要方法:
- 在各种草鱼组织中进行基因克隆和表达分析.
- 细胞局部化研究 (线粒体,全细胞).
- 功能性测试涉及鱼细胞和大肠杆菌中的基因/蛋白质表达,以评估对H2O2,重金属和GCRV感染的耐药性.
- DNA 保护测定.DNA 保护测定.
- 自诱导和抑制实验. 自诱导和抑制实验.
主要成果:
- 成功克隆了CiPrx5和CiPrx6基因,并显示出无处不在的组织表达,其水平因GCRV暴露而调节.
- CiPrx5定位在线粒体中,而CiPrx6在整个细胞中被发现.
- 过度表达CiPrx5和CiPrx6增强了对氧化应激 (H2O2,重金属) 的抵抗力,并保护了DNA免受损伤.
- 这两种过氧素都抑制了GCRV复制,降低了ROS水平,并在感染的鱼细胞中诱导了自.
- 抗病毒和ROS缓解作用依赖于自,因为它们被自抑制剂取消.
结论:
- 草 Prx5 和 Prx6 在对抗氧化应激和GCRV感染方面发挥着重要作用.
- 这些过氧素通过促进自来发挥其保护作用,这反过来又抑制病毒复制并减少细胞内ROS.
- CiPrx5和CiPrx6是增强水产养殖中抗病能力的有希望的目标.
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