来自Physalis angulate活性提取物的维他诺化物及其抗肝纤维化作用
Fu-Rui Wang1, Mei-Lin Peng2, Qin-Feng Zhu3
1State Key Laboratory of Functions and Applications of Medicinal Plants & School of Pharmacy, Guizhou Medical University, Gui-an New District, 550025, Guizhou, China; University Engineering Research Center for the Prevention and Treatment of Chronic Diseases by Authentic Medicinal Materials in Guizhou Province, Gui-an New District, 550025, Guizhou, China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guiyang, 550004, Guizhou, China.
这项研究确定了来自Physalis angulata L.的维他诺化物,具有显著的抗肝纤维化作用. 化合物physalin F通过调节PI3K/AKT/mTOR通路显示出治疗肝纤维化的前景.
科学领域:
- 自然产品化学 自然产品化学
- 药理学 药理学是指药理学的学科.
- 肝病学 肝病学是一种肝病学.
背景情况:
- (Physalis angulata L.) 是一种传统的中医药,用于治疗肝脏疾病.
- 它在治疗肝脏疾病方面的潜力值得科学研究.
研究的目的:
- 调查P. angulata的维他诺化物对抗肝纤维化的活性.
- 阐明活性化合物的作用机制.
主要方法:
- 使用染色学和HPLC进行生物试验-分子网络引导的维他诺化物分离.
- 通过NMR,ECD计算和其他光谱技术来阐明结构.
- 在体外测试 (MTT,AO/EB,伤口愈合,ELISA,西部斑) 和分子对接以评估抗纤维菌效应和机制.
主要成果:
- 分离了8种新的维他诺化物 (withagulides A-H) 和28种已知的维他诺化物.
- 几种维他诺化物显著抑制了I型原蛋白α1 (COL1A1) 表达.
- 在LX-2细胞中,Physalin F显著降低了LX-2细胞中的原I和α-SMA,通过PI3K/AKT/mTOR通路作用.
结论:
- 维他诺化物是抗肝纤维化疗法的有前途的自然产品类别.
- 菲萨林F是肝纤维化的潜在治疗候选者.
- 这项研究为P. angulata在治疗肝纤维化的使用提供了基础.
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