Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Telomeres and Telomerase02:41

Telomeres and Telomerase

23.3K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
23.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells.

Biomolecules·2025
Same author

Editorial: Chronic stress, telomeres and aging.

Frontiers in endocrinology·2024
Same author

Antioxidant Effects of Cactus Seed Oil against Iron-Induced Oxidative Stress in Mouse Liver, Brain and Kidney.

Molecules (Basel, Switzerland)·2024
Same author

Author Correction: The cellular level of telomere dysfunction determines induction of senescence or apoptosis in vivo.

EMBO reports·2024
Same author

Role of Telomeres and Telomerase in Cancer and Aging.

International journal of molecular sciences·2023
Same author

The Telomerase Connection of the Brain and Its Implications for Neurodegenerative Diseases.

Stem cells (Dayton, Ohio)·2022

相关实验视频

Updated: Jul 4, 2025

Droplet Digital TRAP ddTRAP: Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
06:38

Droplet Digital TRAP ddTRAP: Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

Published on: May 3, 2019

8.9K

使用TRAP试验测量端粒酶活性.

Gabriele Saretzki1

  • 1Biosciences Institute, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne, United Kingdom.

Methods in cell biology
|February 1, 2024
PubMed
概括

端粒酶重复放大协议 (TRAP) 测量端粒酶活性,对于癌症诊断和治疗至关重要. 本指南详细介绍了TRAP方法,强调了控制和解释,以避免这种复杂技术的陷.

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 癌症研究 癌症研究

背景情况:

  • 端粒酶是一种逆转录酶 (TERT/TERC),在染色体末端合成端粒.
  • 端粒酶活性在生殖细胞,干细胞和癌细胞中至关重要.
  • 检测端粒酶活性 (TA) 对癌症诊断和治疗至关重要.

研究的目的:

  • 描述用于检测端粒酶活性 (TA) 的各种端粒酶重复放大协议 (TRAP) 方法.
  • 为执行和解释TRAP测定提供指导,包括基于凝和ELISA的方法.
  • 突出控制和样本准备对于成功应用TRAP测试的重要性.

主要方法:

  • 基于凝的TRAP分析的详细描述,包括优点和局限性.
  • 解释如何进行基于酶链接免疫吸收试验 (ELISA) 的TRAP试验.
  • 简要提到先进的TRAP修改,如实时PCR,同热放大,纳米技术和基于CRISPR/Cas的方法.

主要成果:

  • 通过TRAP测试,可以检测各种细胞类型中的端粒酶活性.
  • 本章提供了对测试执行和结果解释的实用见解.
  • 为了成功地应用TRAP,需要对控制和样本准备进行仔细的注意.
关键词:
检测 检测 检测 检测 检测艾丽莎·伊丽莎·伊丽莎测量方法 测量方法这是一个PCRPCR.这是TRAP测试.端粒酶的活性活动.

更多相关视频

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

Published on: September 2, 2014

14.7K
Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

2.9K

相关实验视频

Last Updated: Jul 4, 2025

Droplet Digital TRAP ddTRAP: Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
06:38

Droplet Digital TRAP ddTRAP: Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

Published on: May 3, 2019

8.9K
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

Published on: September 2, 2014

14.7K
Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

2.9K

结论:

  • TRAP测定是癌症临床诊断和评估向端粒酶的抗癌疗法的必不可少的工具.
  • 了解TRAP方法,包括潜在的陷,对于可靠的结果至关重要.
  • 本章是研究人员和临床医生的各种TRAP技术的实用指南.